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Last update: doc. RNDr. Josef Cvačka, Ph.D. (27.11.2023)
After completing the course, students will describe the mass spectrometer and the functions of its basic components. They will explain the principles of ionization, mass analysis, and ion detection. They will clarify how to use mass spectrometry and mass spectrometric detection for different types of analytes and analytical problems. They will describe the signals in the mass spectrum and explain the spectrum given the structural formula of the analyte. They will apply the rules of interpretation and determine or estimate an unknown substance's structure based on the mass spectrum. |
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Last update: doc. RNDr. Karel Nesměrák, Ph.D. (28.10.2019)
Mass Spectrometry – A Textbook, 3rd edition, Gross, Jürgen H., Springer Heidelberg, 2017 |
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Last update: doc. RNDr. Josef Cvačka, Ph.D. (27.11.2023)
The course ends with credit and an exam. |
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Last update: doc. RNDr. Josef Cvačka, Ph.D. (27.11.2023)
2. Principles of ion formation. Electrospray ionization. Atmospheric pressure chemical ionization and photoionization. Electron and chemical ionization. Matrix-assisted laser ionization/desorption. 3. Excursion to an external workplace dealing with mass spectrometry. 4. Movement of ions in electric and magnetic fields. Time of flight analyzer. Quadrupole analyzer. Ion trap. Orbital trap. 5. Ion fragmentation (CID), tandem MS, triple quadrupole, and hybrid instruments. Ion detection and vacuum technology. 6. Practical aspects - choice of experiment conditions, operation, and maintenance of spectrometers. 7. Interpretation of mass spectra of small molecules. 8. Interpretation of mass spectra of small molecules. 9. Quantifying small molecules using GC/MS, LC/MS, and CE/MS. 10. LC/MS of biomolecules: Proteins, antibodies. 11. Data processing and interpretation in proteomics. |