SubjectsSubjects(version: 957)
Course, academic year 2024/2025
   Login via CAS
Analytical Mass Spectrometry - MC230P78
Title: Analytická hmotnostní spektrometrie
Czech title: Analytická hmotnostní spektrometrie
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 3
Examination process: summer s.:combined
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: cancelled
Language: Czech
Note: enabled for web enrollment
Guarantor: doc. RNDr. Ivan Jelínek, CSc.
RNDr. Martin Štícha, Ph.D.
Is incompatible with: MC230P29
Opinion survey results   Examination dates   Schedule   
Annotation -
The lecture is designated for pre gradual and post gradual students specialized on analytical chemistry. In the introductory part, the students are get acquainted with fundamental construction parts of mass spectrometer and common instrumentation used in analytical practice. The following part deals with the fundamentals of the interpretation of mass-spectra of organic compounds. Individual fragmentation mechanisms are explained and discussed in connection with real electron impact mass spectra.
Last update: Jelínek Ivan, doc. RNDr., CSc. (25.10.2019)
Literature -

Spektrometrické analytické metody II., I. Němcová, P. Engst, I. Jelínek, J. Sejbal, P. Rychlovský, Karolinum, Praha 1998 (Lecture notes).

Interpretation of Mass Spectra, F. W. McLafferty, University Science Books, Mill Valley, CA 94941, 1980.

On-line  data are not available.

Last update: Jelínek Ivan, doc. RNDr., CSc. (25.10.2019)
Requirements to the exam -

Exam rules

Taking the written test covering lectured problematics.

Last update: Jelínek Ivan, doc. RNDr., CSc. (25.10.2019)
Syllabus -

Introduction. Experimental setup of mass spectrometer. The survey of ionization techniques. Mass analyzers. Magnetic sector. Electric sector. Quadrupole mass analyzer. Ion trap. Time of flight mass analyzer. Detectors. Multiplier detectors. Scintillation photomultiplier detectors.

Mass spectrum. Molecular ion. Analysis of isotopic composition. Stable isotopes. Ring + double bonds rule.

Theory of fragmentation. Charge and unpaired electron localization in molecular ion. Fragmentation of molecular ions, fragmentation pathways. Factors influencing ion intensity. Fragmentation mechanisms of organic molecules. Sigma bond cleavage. Alpha cleavage initiated by radical site. Cleavage initiated charge site. Cleavage of cyclic structures. Rearrangement reactions. Rearrangements initiated by radical site. Rearrangements initiated by charge site.

Mass spectra of simple organic compounds and their interpretation.

Last update: Jelínek Ivan, doc. RNDr., CSc. (25.10.2019)
 
Charles University | Information system of Charles University | http://www.cuni.cz/UKEN-329.html