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Last update: doc. RNDr. Karel Nesměrák, Ph.D. (28.10.2019)
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Last update: doc. RNDr. Karel Nesměrák, Ph.D. (28.10.2019)
1. National Human Genome Institute http://www.genome.gov/Education/ |
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Last update: doc. RNDr. Karel Nesměrák, Ph.D. (28.10.2019)
The exam from Genomic analysis in clinical practice is written. The requirements for the exam are given by the extent of the lecture. At least 68% success rate is required to pass the exam. |
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Last update: doc. RNDr. Marek Minárik, Ph.D. (09.09.2021)
2. Fluorescence in genomic analysis: intercalation, FRET, quenching, quantum dots (theory, applications) 3. Separation of DNA/RNA: Electromigration methods (theory, gel, capillary and microchip electrophoresis), Chromatographic methods (ion, reversed phase and ion-pair HPLC) 4. Enzymatic methods in DNA/RNA analysis: polymerases, restrictases, ligases (properties, usage) 5. PCR methods: general principles, optimization, modification, real-time PCR (Ct, quantification, calibration), digital PCR, emulsion and bridge PCR 6. Mutation and mutation analysis: point, chromosomal, mutation vs. mutation. polymorphisms, sensitivity, specificity, scanning vs genotyping, haplotypes, GWAS, applications 7. Sequencing I. and fragmentation analysis: history, Sanger, pyrosequencing, STR markers (genetic identity and paternity determination), prenatal diagnostics, MSI, LOH 8. Sequencing II .: NGS / MPS, libraries, instrumentation, targeted / deep sequences, coverage, applications 9. Molecular oncology: origin and course of cancer, epidemiology, diagnostics, staging, treatment modalities, prediction, prognosis 10. Genomic analysis in oncology: cell signaling, oncogenes, tumor suppressors, epigenomics (DNA methylation, miRNA), molecular markers 11. New trends |