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Course, academic year 2022/2023
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Analytical Mass Spectrometry - MC230P29
Title: Analytická hmotnostní spektrometrie
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Guarantor: RNDr. Martin Štícha, Ph.D.
Teacher(s): RNDr. Martin Štícha, Ph.D.
Incompatibility : MC230P78
Annotation -
Last update: OPEKAR (23.04.2002)
Special course for pre-graduate and graduate students specialized in analytical chemistry. In an introductory part the students are familiarized with fundamental construction schemes of mass-spectrometer. The survey of mass spectrometric instrumentation used at present is given. The following part is aimed at the interpretation of electron impact mass-spectra of organic comounds. The fundamentals of ion fragmentation are discussed. Theoretical considerations are accompanied with the examples of real mass-spectra and the explanation of important fragmentation pathways.
Literature - Czech
Last update: doc. RNDr. Ivan Jelínek, CSc. (22.09.2014)

Spektrometrické analytické metody II., I. Němcová, P. Engst, I. Jelínek, J. Sejbal, P. Rychlovský, Karolinum, Praha 1998 (Skriptum).

Interpretation of Mass Spectra, F. W. McLafferty, University Science Books, Mill Valley, CA 94941, 1980.

Elektronické podklady nejsou dostupné

Requirements to the exam - Czech
Last update: doc. RNDr. Ivan Jelínek, CSc. (05.12.2011)

Podmínky pro udělení zkoušky:

Úspěšné absolvování písemného testu v rozsahu tématických okruhů přednášek a základní studijní literatury.

Syllabus -
Last update: OPEKAR (29.01.2003)

Introduction. Experimental design of mass spectrometer. Survey of ionization techniques. Electron impact ionization. Chemical ionization. Other ionization techniques. Mass analyzers. Magnetic mass analyzer. Electric analyzer. Quadrupole analyzer. Ion trap. Time of flight analyzer. Detectors. Multipliers. Scintilation photomultipliers.

Mass spectrum. Molecular ion. Analysis of isotopic composition. Stable izotopes. Determination of number of rings plus double bonds from elemetal formula.

Theory of fragmentation. Charge and unpaired electron localization in molecular ion. Molecular ion fragmentation, fragmentation paths. Mechanisms of fragmetation of organic molecules. Sigma bond dissociation. Alfa cleavage initiated by radical-site. Cleavage initiated by charge-site. Decompositions of cyclic structures. Rearrangements. Radical-site rearrangements. Charge-site rearrangements.

Mass spectra of simple organic molecules and their interpretation.

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