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Course, academic year 2023/2024
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Separation methods - MC230P05N
Title: Separační metody
Czech title: Separační metody
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2020 to 2023
Semester: summer
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:3/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: prof. RNDr. Pavel Coufal, Ph.D.
Teacher(s): prof. RNDr. Pavel Coufal, Ph.D.
RNDr. Jana Sobotníková, Ph.D.
Annotation -
The course of separation methods gives a survey of modern analytical separation techniques. General theory of separations. A summary of the physico-chemical principles like transport phenomena, solubility, adsorption, ion exchange, size exclusion, biological recognition. The theory of chromatography and of electromigration. A systematic description of important separation techniques. The course ends through a written exam.

Learning outcomes: After completing the course, the student will be able to describe modern analytical separation methods, use the general theory of separation, explain physico-chemical separation principles, define single-stage separation methods, clarify the theory of chromatography, explain electromigration, define planar and column chromatography techniques, explain the overview and division of electromigration methods, perform extraction, clarify supercritical fluid extraction, perform paper chromatography, perform thin layer chromatography, perform gas chromatography, perform liquid chromatography, clarify supercritical fluid chromatography and perform capillary electrophoresis.
Last update: Coufal Pavel, prof. RNDr., Ph.D. (05.02.2024)
Literature -

1. ,

2. Analytické separační metody, K. Štulík a kol., Karolinum, Praha, 2005

3. Teoretické základy a separační principy kapilárních elekromigračních metod, V. Kašička, Chemické listy, 91 (1997) 320-329

4. Analytická chemie, Z. Holzbecher, J. Churáček a kol., SNTL, Praha, 1987

5. Instrumentální analýza, F. Čůta, M. Popl a kol., SNTL, Praha, 1986

6. Plynová chromatografie I, II, III, E. Smolková, V. Pacáková, L. Feltl, K. Štulík, skripta PřF UK, Praha, 1976

7. Vysokoúčinná kapalinová chromatografie, V. Pacáková, K. Štulík, skripta PřF UK, Praha, 1986

8. Chromatography today, C.F. Poole, S.K. Poole, Elsevier, Amsterdam, 1991

Last update: Coufal Pavel, prof. RNDr., Ph.D. (05.02.2024)
Requirements to the exam -

Examination of separation methods is written. The requirements for the exam are given by the extent of the lecture. At least 59% success rate is required to pass the exam.

Last update: Coufal Pavel, prof. RNDr., Ph.D. (05.02.2024)
Syllabus -

1. A survey of modern analytical separation techniques.

2. Theory of separations based on the concepts of differential migration of the analytes.

3. Physico-chemical principles of the underlying processes and interactions, i.e., transport phenomena (diffusion, convection), solubility (precipitation, coprecipitation, dissolution), adsorption, ion exchange, size exclusion, biological recognition.

4. Single step separation methods - precipitation and co-precipitation, electrodeposition, liquid-liquid extraction.

5. The theory of chromatography and of electromigration.

6. Planar and column chromatographic techniques.

7. Paper chromatography (principle, stationary and mobile phases, detection techniques, applications).

8. Gas chromatography - theory of dissolution of gases in liquids, selection of stationary phase, instrumentation for GC - injectors, packed and capillary columns, detectors (TCD, FID, ECD, MS etc.), temperatuer programming, derivatization, pyrolysis GC, applications.

9. High-performance liquid chromatography - adsorption, chromatography on chemically bonded stationary phases,, ion-exchange, exclusion and bioafinity chromatography (principle, selection of mobile phase, aplications). HPLC instrumentation (pumps, injectors, columns, detectors, mobile phase programming). Derivatization, applications.

10. Supercritical fluid extraction and chromatography (principle, selection of stationary and mobile phase, instrumenmtation, aplications).

11. Electromigration techniques overwiev. Electrophoretic migration and mobility. Ecectroosmotic flow and mobility.Electrical double layer and zeta potential. Velocity and temperature profiles. Instrument for CZE. CZE separation mechanism. Electropherogram. Calculation of mobilities. Weak electrolytes. Sample introduction. Detection. Analytical information of electropherogram. Applications.

Last update: Coufal Pavel, prof. RNDr., Ph.D. (05.02.2024)
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