Subjects

This practical laboratory course introduces students to the conventional instrumental techniques for chemical analysis. The analytical methods taught are divided into 3 basic groups: electrochemical, separation, and spectrometric. The course emphasizes understanding the principles demonstrated for each instrumental method. Students are guided through the process of handling raw experimental data, its statistical evaluation and presenting calculated results. Strict compliance with GLP and safety regulations is required throughout the course. Upon completion of the course, students should be able to work independently with common analytical instruments. An integral part of the course and its classification involves continuously controlling the knowledge required to perform each task, classifying protocols and evaluating laboratory work.

This laboratory course is organized in two-week blocks, with students working in small groups. It is offered in the winter and summer semesters. In the winter semester, the course is organized at the beginning of October.

Last update: Sobotníková Jana, RNDr., Ph.D. (21.07.2025)

1. Task Instructions and Safety Regulations can be downloaded from SIS.

2. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, 7th ed., 2017
3. D. C. Harris, Quantitative Chemical Analysis, 10th ed., 2019
4. D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch, Fundamentals of Analytical Chemistry, 10th ed., 2022

Last update: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)

This course is credited with a mark (MC). Marked credit will be awarded upon completion of all course tasks and submission of all protocols within 14 days of the course ending.

Protocols will be corrected and graded on a scale of 1 (excellent, A) to 4 (unsatisfactory, F) according to their quality (results, statistical analysis, and presentation). When correcting protocols, great importance is given to the correctness of the calculated values, the correct indication of the units of the quantities, and the completeness of the submitted protocol. If the protocol is incomplete or contains serious errors, it will be graded with a 4 (F) and it is the student's responsibility to correct all errors and resubmit the corrected protocol, including the original protocol, within 2 weeks. The revised protocol will be re-evaluated, and both marks of the revised report will be included in the overall course grade. If, during the correction process, the teacher finds evidence of plagiarism (verbatim use of text or values from another report), the report will automatically be marked 4/4 without the possibility of further correction.

The final mark for the course is calculated using an average of the marks for oral knowledge verification before each task and for graded protocols.

To successfully pass the course means to achieve the final mark of excellent, very good, or good (A, B, C, D, or E).

Last update: Sobotníková Jana, RNDr., Ph.D. (04.08.2025)

The following exercises are done:

1. Classical DC polarography
2. Karl Fischer titrations (KF)
3. Spectrophotometry - Determination of dissociation constant of an acid-base indicator
4. Determination of Bi³⁺ and Cd²⁺ ions with AAS and K⁺ ion using Flame photometry
5. Gas chromatography (GC) - Qualitative and quantitative analysis
6. Determination of azodyes in a mixture using RP-HPLC method with spectrophotometric detection
7. Conductometry and Conductometric titration
8. Capillary zone electrophoresis (CZE)

Last update: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)

Upon completion of the course, the student will be able to:

- explain the principle of the above analytical methods

- independently prepare solutions and use instruments (carry out measurements) based on task instructions

- interpret measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves)

- describe, evaluate, and draw conclusions from measured data

- record the procedure, results, and conclusions in a report

for these instrumental analytical methods (polarography, conductometry and conductometric titration, Karl Fischer titration, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection.

Last update: Sobotníková Jana, RNDr., Ph.D. (21.07.2025)

Entry requirements include knowledge of basic chemical calculations, such as quantifying chemical equations, calculating concentration and percentage content, and preparing solutions by dilution. Candidates should also be familiar with calibration curve and standard addition principles, as well as the instrumental analytical methods used in the scheduled tasks.

Last update: Sobotníková Jana, RNDr., Ph.D. (17.07.2025)

It cannot be enrolled at the same time as MC230C13 and MC230C15.

Last update: Sobotníková Jana, RNDr., Ph.D. (04.02.2025)

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