Advanced Laboratory Course in Analytical Chemistry - MC230C21E
Title: Advanced Laboratory Course in Analytical Chemistry
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2024
Semester: both
E-Credits: 6
Hours per week, examination: 0/2, C [TS]
Extent per academic year: 2 [weeks]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: English
Note: priority enrollment if the course is part of the study plan
you can enroll for the course in winter and in summer semester
Guarantor: RNDr. Jana Sobotníková, Ph.D.
Teacher(s): RNDr. Václav Červený, Ph.D.
RNDr. Hana Dejmková, Ph.D.
Anton Korban, Ph.D.
RNDr. Eliška Nováková, Ph.D.
RNDr. Jana Sobotníková, Ph.D.
prof. RNDr. Vlastimil Vyskočil, Ph.D.
Incompatibility : MC230C10, MC230C13, MC230C15
Is incompatible with: MC230C15, MC230C10, MC230C13
Opinion survey results   Examination dates   WS schedule   SS schedule   
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download Rules_Advanced Laboratory Course in Analytical Chemistry MC230C21E.pdf Rules and organisation of teaching of the Advanced Laboratory Course in Analytical Chemistry RNDr. Jana Sobotníková, Ph.D.
Annotation
This practical laboratory course introduces students to the conventional instrumental techniques of chemical analysis. The analytical methods taught are divided into 3 basic groups: electrochemical, separation and spectrometric methods. Emphasis is placed on understanding the principles demonstrated for each instrumental method. Students are guided through the handling of raw experimental data, its statistical evaluation and the presentation of calculated results. Strict compliance with GLP and safety regulations is required throughout the course. Upon completing the course, students should be able to work independently with common analytical instruments. An integral part of the classification is the continuous control of the knowledge required to perform each task, the classification of protocols, and the evaluation of laboratory work.

This laboratory course is organized in two-week blocks with students working in small groups. This laboratory course is organized in the summer and winter terms.
Last update: Sobotníková Jana, RNDr., Ph.D. (04.02.2025)
Literature

1. Task Instructions and Safety Regulations can be downloaded from SIS.

2. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, 7th ed., 2017
3. D. C. Harris, Quantitative Chemical Analysis, 10th ed., 2019
4. D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch, Fundamentals of Analytical Chemistry, 10th ed., 2022

Last update: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)
Requirements to the exam

Credits will be awarded on completion of all prescribed tasks in the course and submission of all properly prepared protocols within 14 days of the end of the course.

Protocols will be corrected and graded on a scale of 1 (excellent, A) to 4 (unsatisfactory, F) according to their quality (results, statistical analysis, and presentation). When correcting protocols, great importance is given to the correctness of the calculated values, the correct indication of the units of the quantities, and the completeness of the submitted protocol. If the protocol is incomplete or contains serious errors, it will be graded with a 4 (F) and it is the student's responsibility to correct all errors and resubmit the corrected protocol, including the original protocol, within 2 weeks. The revised protocol will be re-evaluated and both grades of the revised report will be included in the overall course grade. If, when correcting the report, the teacher finds evidence of plagiarism (verbatim use of text or values from another report), such a report will automatically be marked 4/4 without the possibility of further correction.

Credits will be awarded to students whose sum of all grades from oral examinations (including 4 and grades from corrective examinations) does not exceed 25 and whose sum of all grades from protocols (also including 4 and grades from revised protocols) does not exceed 25.

If the sum of the grades from the oral examinations or protocols exceeds 25, an additional oral examination will be required for credit.

If the sum of the grades from the oral examinations or protocols exceeds 30, no course credit is awarded. Re-enrolment in the course is required to receive credit.

Last update: Sobotníková Jana, RNDr., Ph.D. (04.02.2025)
Syllabus

The following exercises are done:

1. Classical DC polarography
2. Karl Fischer titrations (KF)
3. Spectrophotometry - Determination of dissociation constant of an acid-base indicator
4. Determination of  Bi3+ and Cd2+ ions with AAS and K+ ion using Flame photometry
5. Gas chromatography (GC) - Qualitative and quantitative analysis
6. Determination of azodyes in a mixture using RP-HPLC method with spectrophotometric detection
7. Conductometry and Conductometric titration
8. Capillary zone electrophoresis (CZE)

Last update: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)
Learning outcomes

On completion of the course, the student will be able to: for these instrumental analytical methods (polarography, conductometry and conductometric titration, Karl Fischer titration, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):

- explain the principle of the above analytical methods

- independently prepare solutions and use instruments (carry out measurements) based on task instructions

- interpret measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves)

- describe, evaluate and draw conclusions from measured data

- record the procedure, results and conclusions in a report

Last update: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)
Entry requirements

Students MUST know about the principles of electrochemical, separation, and spectrometric analytical methods. In addition, the evaluation of raw experimental data, their statistical evaluation, and the presentation of calculated results are necessary input requirements for attending this course. 

Last update: Sobotníková Jana, RNDr., Ph.D. (04.02.2025)
Registration requirements

It cannot be enrolled at the same time as MC230C13 and MC230C15.

Last update: Sobotníková Jana, RNDr., Ph.D. (04.02.2025)