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Last update: RNDr. Jakub Hraníček, Ph.D. (27.09.2023)
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Last update: RNDr. Jakub Hraníček, Ph.D. (27.09.2023)
Basic study literature: 1) I. Nemcova, L.Cermakova, P.Rychlovsky, Spectrometric analytical methods I., Karolinum, Prague 1997. 2) I. Nemcova, P.Engst, I.Jelinek, J.Sejbal, P.Rychlovsky, Spectrometric Analytical Methods II., Karolinum, Prague 1998 Additional and recommended literature: 3) Welz B., Sperling M., Atomic Absorption Spectrometry, Third Edition, 1999, WILEY-VCH Verlag GmbH 4) Dedina J., Tsalev D. L.: Hydride generation atomic absorption spectrometry 1995, John Wiley and Sons, Inc. 5) Encyclopedia of Analytical Chemistry, 1999-2014, by John Wiley and Sons, Inc. Chapter: Generation of Volatile Compounds for Analytical Atomic Spectroscopy by Jiří Dědina 6) McNair H.M., Miller J.M .: Basic Gas Chromatography, 1999-2014, John Wiley & amp; 7) Jennings W., Mittlefehldt E., Stremple P .: Analytical Gas Chromatography, Second Edition, 1997, John Wiley & amp; |
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Last update: RNDr. Jakub Hraníček, Ph.D. (27.09.2023)
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Last update: RNDr. Jakub Hraníček, Ph.D. (27.09.2023)
The practice of advanced spectrometric methods currently consists of three compulsory tasks (solved in any order), which are carried out in the laboratories of the Department of Analytical Chemistry at Charles University and in the Laboratories of the Department of Trace Element Analysis, Czech Academy of Sciences. Individual tasks will be realized in terms and times given by each supervisor. List of tasks Kinetic assays with spectrophotometric detection. (a task for 2.5 hours) Determination of total mercury content (AMA-254) and speciation analysis (HPLC-ICP-MS) in biological matrix samples. (a task for 5-6 hours) * Determination of structure of organic compounds - NMR spectroscopy. (a task for 2 hours) * Task 2 will be realized at the Institute of Analytical Chemistry of the ASCR (see below for contact details). In the practice of advanced spectrometric methods, kinetic determination with UV / Vis detection technique will be demonstrated to determine trace amounts of copper. In another complex task, students will learn the methods of HPLC-ICP-MS and AAS, as well as sample pre-treatment. First, mercury species will be extracted from both the sample and the reference material. Subsequently, the content of inorganically bound mercury and methylmercury in the extract will be determined by HPLC-ICP-MS. The results will be compared with the determination of total mercury content without any pre-treatment of the sample on a dedicated atomic absorption spectrometer AMA-254. The last compulsory task of the practical exercise will be devoted to the use of NMR spectrometry for the identification of organic compounds based on 1H spectra. |
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Last update: RNDr. Jakub Hraníček, Ph.D. (27.09.2023)
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Last update: RNDr. Jakub Hraníček, Ph.D. (27.09.2023)
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