Laboratory Course on Separation Methods - MC230C16
Title: Praktikum ze separačních metod
Czech title: Praktikum ze separačních metod
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2023
Semester: both
E-Credits: 4
Hours per week, examination: 0/2, C [DS]
Capacity: 20
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: specialized
Note: enabled for web enrollment
priority enrollment if the course is part of the study plan
you can enroll for the course in winter and in summer semester
Guarantor: doc. RNDr. Tomáš Křížek, Ph.D.
Teacher(s): prof. RNDr. Zuzana Bosáková, CSc.
Anton Korban, Ph.D.
doc. RNDr. Petr Kozlík, Ph.D.
doc. RNDr. Tomáš Křížek, Ph.D.
Opinion survey results   Examination dates   WS schedule   
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download HPLC Chirální separace2012.pdf prof. RNDr. Zuzana Bosáková, CSc.
Annotation -
The laboratory course on separation methods is focused on gaining theoretical knowledge as well as experimental skills in advanced analytical instrumentation. Students will solve four complex problems using high-performance liquid chromatography with tandem mass detection, gas chromatography with mass or flame ionization detection (student's choice), capillary zone electrophoresis, and chiral capillary electrophoresis.

After passing this course students will be able in capillary electrophoresis, liquid chromatography with tandem mass spectrometry, and gas chromatography with flame-ionization or mass detection:
Independently operate instruments and perform analyses on the basic user level with written instructions.
Evaluate and interpret the experimental records.
Draw appropriate conclusions from the results.
Create a concise report containing a description of the experimental work, results, and conclusions, without serious factual or formal errors.
Last update: Křížek Tomáš, doc. RNDr., Ph.D. (09.01.2024)
Literature -

Analytické separační metody, K. Štulík a kol., Karolinum, Praha, 2005.
Encyclopedia of Separation Science. M. Crooke, C. F. Poole (Eds.) Academic Press 2000
Chromatography today, C.F. Poole, S.K. Poole, Elsevier, Amsterdam, 1991.

Last update: Nesměrák Karel, doc. RNDr., Ph.D. (28.10.2019)
Requirements to the exam -
Participation in the practical exercises, submission of appropriate reports, examinations for individual tasks, and scoring minimum 60% in the final test.
Last update: Křížek Tomáš, doc. RNDr., Ph.D. (05.09.2023)
Syllabus -

1) Three amino acids will be separated using a cyclodextrin-based chiral selector by capillary electrophoresis. The migration order of individual enantiomers will be determined. Enantiomers will be quantified using the standard addition method.
2) An HPLC-MS-MS method for analysis of selected organic pollutants will be developed. Focus will be on the optimization of MS/MS detection.
3) This exercise comprises gas chromatography with flame ionization or mass spectrometry detection. Students can choose according to their interests.
4) Ammonium, sodium and potassium ions in urine are separated and determined using capillary electrophoresis. 

Last update: Křížek Tomáš, doc. RNDr., Ph.D. (05.09.2023)