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Course, academic year 2023/2024
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Laboratory Course in Instrumental Analysis - MC230C15
Title: Praktikum z přístrojové analýzy
Czech title: Praktikum z přístrojové analýzy
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2023 to 2023
Semester: summer
E-Credits: 4
Examination process: summer s.:
Hours per week, examination: summer s.:0/3, C [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Note: enabled for web enrollment
Guarantor: RNDr. Jana Sobotníková, Ph.D.
Teacher(s): RNDr. Václav Červený, Ph.D.
RNDr. Hana Dejmková, Ph.D.
RNDr. Eliška Nováková, Ph.D.
prof. RNDr. František Opekar, CSc.
RNDr. Jana Sobotníková, Ph.D.
prof. RNDr. Vlastimil Vyskočil, Ph.D.
Annotation -
This practical laboratory course introduces students to the conventional instrumental techniques of chemical analysis. The analytical methods taught are divided into 3 basic groups: electrochemical, separation and spectrometric methods. Emphasis is placed on understanding the principles demonstrated for each instrumental method. Students are guided through the handling of raw experimental data, its statistical evaluation and the presentation of calculated results. Strict compliance with GLP and safety regulations is required throughout the course. Upon completion of the course, students should be able to work independently with common analytical instruments. An integral part of the classification is the continuous control of the knowledge required to perform each task, the classification of protocols and the evaluation of laboratory work.

On completion of the course, the student will be able to: for these instrumental analytical methods (polarography, conductometry and conductometric titration, Karl Fischer titration, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):
- explain the principle of the above analytical methods
- independently prepare solutions and use instruments (carry out measurements) on the basis of task instructions
- interpret measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves)
- describe, evaluate and draw conclusions from measured data
- record the procedure, results and conclusions in a report

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Literature -

1. Task Instructions and Safety Regulations can be downloaded from SIS.

2. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, 7th ed., 2017
3. D. C. Harris, Quantitative Chemical Analysis, 10th ed., 2019
4. D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch, Fundamentals of Analytical Chemistry, 10th ed., 2022

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Requirements to the exam -

Credits will be awarded on completion of all prescribed tasks in the course and submission of all properly prepared protocols within 14 days of the end of the course.

Protocols will be graded from 1 (excellent) to 4 (unsatisfactory) according to their quality (results, statistical analysis and presentation).

The sum of the scores of the graded protocols must not exceed the critical limit (25) to receive the credits.

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Syllabus -

Following exercises are done:

1. Classical DC polarography
2. Karl Fischer titrations (KF)
3. Spectrophotometry - Determination of dissociation constant of an acid-base indicator
4. Determination of  Bi3+ and Cd2+ ions with AAS and K+ ion using Flame photometry
5. Gas chromatography (GC) - Qualitative and quantitative analysis
6. Determination of azodyes in a mixture using RP-HPLC method with spectrophotometric detection
7. Conductometry and conductometric titration
8. Capillary zone electrophoresis (CZE)

Last update: Sobotníková Jana, RNDr., Ph.D. (07.09.2021)
Entry requirements -

Students should have knowledge about principles of electrochemical, separation and spectrometric analytical methods. Also evaluation of raw experimental data, their statistical evaluation and presentation of calculated results are necessary input requirements for attending this course. 

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Registration requirements -

Cannot be enrolled at the same time as MC230C13, MC230C10 and MC230C21E.

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
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