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This practical laboratory course introduces students to the conventional instrumental techniques of chemical analysis. The analytical methods taught are divided into 3 basic groups: electrochemical, separation and spectrometric methods. Emphasis is placed on understanding the principles demonstrated for each instrumental method. Students are guided through the handling of raw experimental data, its statistical evaluation and the presentation of calculated results. Strict compliance with GLP and safety regulations is required throughout the course. Upon completion of the course, students should be able to work independently with common analytical instruments. An integral part of the classification is the continuous control of the knowledge required to perform each task, the classification of protocols and the evaluation of laboratory work.
On completion of the course, the student will be able to: for these instrumental analytical methods (polarography, conductometry and conductometric titration, Karl Fischer titration, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection): - explain the principle of the above analytical methods - independently prepare solutions and use instruments (carry out measurements) on the basis of task instructions - interpret measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves) - describe, evaluate and draw conclusions from measured data - record the procedure, results and conclusions in a report Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
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1. Task Instructions and Safety Regulations can be downloaded from SIS. 2. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, 7th ed., 2017 Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
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Credits will be awarded on completion of all prescribed tasks in the course and submission of all properly prepared protocols within 14 days of the end of the course. Protocols will be graded from 1 (excellent) to 4 (unsatisfactory) according to their quality (results, statistical analysis and presentation). Credits will be awarded to students whose sum of all grades from oral examinations (including 4 and grades from corrective examinations) does not exceed 25 and whose sum of all grades from protocols (also including 4 and grades from revised protocols) does not exceed 25. If the sum of the grades from the oral examinations or protocols exceeds 25, an additional oral examination will be required for credit. If the sum of the grades from the oral examinations or protocols exceeds 30, no course credit can be awarded. Re-enrolment in the course is required to receive credit. Last update: Sobotníková Jana, RNDr., Ph.D. (22.01.2025)
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Following exercises are done: Last update: Sobotníková Jana, RNDr., Ph.D. (07.09.2021)
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Students should have knowledge about principles of electrochemical, separation and spectrometric analytical methods. Also evaluation of raw experimental data, their statistical evaluation and presentation of calculated results are necessary input requirements for attending this course. Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
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Cannot be enrolled at the same time as MC230C13, MC230C10 and MC230C21E. Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
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