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This practical laboratory course introduces students to the conventional instrumental techniques for chemical analysis. The analytical methods taught are divided into 3 basic groups: electrochemical, separation, and spectrometric. The course emphasizes understanding the principles demonstrated for each instrumental method. Students are guided through the process of handling raw experimental data, its statistical evaluation and presenting calculated results. Strict compliance with GLP and safety regulations is required throughout the course. Upon completion of the course, students should be able to work independently with common analytical instruments. An integral part of the course and its classification involves continuously controlling the knowledge required to perform each task, classifying protocols and evaluating laboratory work.
This laboratory course is organized in two-week sessions, with students working in small groups. It takes place at the beginning of October (2-WEEK BLOCK). FOR SUCCESSFUL COMPLETION OF THE PRACTICAL TRAINING, IT IS RECOMMENDED THAT STUDENTS COMPLETE MG431P99 ANALYTICAL METHODS IN GEOCHEMISTRY OR MC230P85 ANALYTICAL CHEMISTRY I+II COURSES. On completion of the course, the student will be able to: - explain the principle of the above analytical methods - independently prepare solutions and use instruments (carry out measurements) based on task instructions - interpret measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves) - describe, evaluate, and draw conclusions from measured data - record the procedure, results, and conclusions in a report for these instrumental analytical methods (polarography, conductometry and conductometric titration, Karl Fischer titration, atomic absorption and emission spectrometry, spectrophotometry, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection). Last update: Sobotníková Jana, RNDr., Ph.D. (21.07.2025)
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1. Instructions for tasks, PDF file can be downloaded in SIS Last update: Sobotníková Jana, RNDr., Ph.D. (14.02.2024)
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Credits will be awarded upon completion of all prescribed tasks in the course and submission of all properly prepared protocols within 14 days of the end of the course. Protocols will be corrected and graded on a scale of 1 (excellent, A) to 4 (unsatisfactory, F) according to their quality (results, statistical analysis, and presentation). When correcting protocols, great importance is given to the correctness of the calculated values, the correct indication of the units of the quantities, and the completeness of the submitted protocol. If the protocol is incomplete or contains serious errors, it will be graded with a 4 (F) and it is the student's responsibility to correct all errors and resubmit the corrected protocol, including the original protocol, within 2 weeks. The revised protocol will be re-evaluated, and both grades of the revised report will be included in the overall course grade. If, during the correction process, the teacher finds evidence of plagiarism (verbatim use of text or values from another report), the report will automatically be marked 4/4 without the possibility of further correction. Credits will be awarded to students whose sum of all grades from oral examinations (including 4 and grades from corrective examinations) does not exceed 25 and whose sum of all grades from protocols (also including 4 and grades from revised protocols) does not exceed 25. If the sum of the grades from the oral examinations or protocols exceeds 25, an additional oral examination will be required for credit. If the sum of the grades from the oral examinations or protocols exceeds 30, no course credit is awarded. Re-enrolment in the course is required to receive credit. Last update: Sobotníková Jana, RNDr., Ph.D. (17.07.2025)
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The following tasks are performed: 1. Classical DC polarography: determination of Cd2+ in the sample using the standard addition method Instructions for tasks are available in SIS Last update: Sobotníková Jana, RNDr., Ph.D. (14.02.2024)
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On completion of the course, the student will be able to: Last update: Sobotníková Jana, RNDr., Ph.D. (17.07.2025)
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Entry requirements include knowledge of basic chemical calculations, such as quantifying chemical equations, calculating concentration and percentage content, and preparing solutions by dilution. Candidates should also be familiar with calibration curves and standard addition principles, as well as the instrumental analytical methods used in the scheduled tasks.
Last update: Sobotníková Jana, RNDr., Ph.D. (17.07.2025)
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