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Course, academic year 2023/2024
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Analytical Chemistry for Geochemistry - Laboratory Course - MC230C10
Title: Moderní metody analytické chemie (geol)
Czech title: Moderní metody analytické chemie (geol)
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2017 to 2023
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:0/2, C [TS]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: RNDr. Jana Sobotníková, Ph.D.
Teacher(s): RNDr. Václav Červený, Ph.D.
RNDr. Hana Dejmková, Ph.D.
RNDr. Eliška Nováková, Ph.D.
prof. RNDr. František Opekar, CSc.
RNDr. Jana Sobotníková, Ph.D.
prof. RNDr. Vlastimil Vyskočil, Ph.D.
Annotation -
This practical laboratory course introduces students to the conventional instrumental techniques of chemical analysis. The analytical methods taught are divided into 3 basic groups: electrochemical, separation and spectrometric methods. Emphasis is placed on understanding the principles demonstrated for each instrumental method. Students are guided through the handling of raw experimental data, its statistical evaluation and the presentation of calculated results. Strict compliance with GLP and safety regulations is required throughout the course. Upon completion of the course, students should be able to work independently with common analytical instruments. An integral part of the classification is the continuous control of the knowledge required to perform each task, the classification of protocols and the evaluation of laboratory work.

This laboratory course is organized in two-week sessions with students working in small groups. This laboratory course takes place at the end of November or in December (2-WEEK BLOCK).


FOR SUCCESSFUL COMPLETION OF THE PRACTICAL TRAINING, IT IS RECOMMENDED THAT STUDENTS COMPLETE MG431P99 ANALYTICAL METHODS IN GEOCHEMISTRY OR MC230P85 ANALYTICAL CHEMISTRY I+II COURSES.

On completion of the course, the student will be able to: for these instrumental analytical methods (polarography, conductometry and conductometric titration, Karl Fischer titration, atomic absorption and emission spectrometry, spectrophotometry, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):
- explain the principle of the above analytical methods
- independently prepare solutions and use instruments (carry out measurements) based on task instructions
- interpret measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves)
- describe, evaluate and draw conclusions from measured data
- record the procedure, results and conclusions in a report

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Literature -

1. Instructions for tasks, PDF file can be downloaded in SIS
2. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, 7th ed., 2017
3. D. C. Harris, Quantitative Chemical Analysis, 10th ed., 2019
4. D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch, Fundamentals of Analytical Chemistry, 10th ed., 2022

Last update: Sobotníková Jana, RNDr., Ph.D. (14.02.2024)
Requirements to the exam -

Credits will be awarded on completion of all prescribed tasks in the course and submission of all properly prepared protocols within 14 days of the end of the course.

Protocols will be graded from 1 (excellent) to 4 (unsatisfactory) according to their quality (results, statistical analysis and presentation).

The sum of the scores of the graded protocols must not exceed the critical limit (25) to receive the credits.

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Syllabus -

The following tasks are performed:

1. Classical DC polarography: determination of Cd2+ in the sample using the standard addition method
2. Conductometry and conductometric titration: determination of hydrochloric acid in the sample
3. Karl Fischer titration: determination of water in methanol and flour samples
4. Atomic absorption and emission spectrometry: determination of Bi3+ and Cd2+ using F-AAS and K+ using flame photometry
5. UV/VIS spectrometry: determination of the dissociation constant of an acid-base indicator
6. High-performance liquid chromatography: determination of azo dyes in a mixture using RP-HPLC
7. Gas chromatography: qualitative and quantitative analysis of hydrocarbon mixtures

Instructions for tasks are available in SIS

Last update: Sobotníková Jana, RNDr., Ph.D. (14.02.2024)
 
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