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This course complets the lecture on Analytical Chemistry and emphasizes mastering the basic operations of chemical analysis, such as weighing techniques, working with volumetric glassware, sample preparation, and dissolution. All work in the laboratory must be carried out safely; therefore, students are familiar with safety protocols and fire regulations for chemical laboratories. The laboratory exercises aim to introduce students to the basic principles of qualitative inorganic analysis and classical and instrumental quantitative analysis methods. This course concludes with a written test and a practical exam.
Students of the individual study programs enroll as follows: WINTER SEMESTER Students of the Bachelor's study program in Clinical and Toxicological Analysis – WS, 2nd year. Students of the Bachelor's study program in Medicinal Chemistry – WS, 3rd year. Students of the Bachelor's study program in Chemistry and Physics of Materials – WS, 3rd year. SUMMER SEMESTER Students of the Bachelor's study program in Environmental Protection – SS, 3rd year. Students of the Bachelor's study program in Biochemistry – SS, 2nd year. Students of the Bachelor's study program in Chemistry – SS, 2nd year. Students of FNSPE (Faculty of Nuclear Sciences and Physical Engineering) – SS, 3rd year. Current information regarding the course will be automatically sent to enrolled students 1-2 weeks before the start of the course. It is assumed that students with specific learning outcomes available in writing, documented in the form of precise assignments, will learn more and at a deeper level during the semester than if these outcomes were absent (as evidenced by evidence-based teaching). According to Government Decree No. 274/2016 Coll. on standards for accreditation in higher education and Rector's Directive 22/2022 of Charles University (Standards of Study Programs at Charles University), the following learning outcomes are supplemented: LEARNING OUTCOMES After completing the course MC230C07N, the student will be able to: Perform basic qualitative analysis operations and demonstrate selected chemical reactions. Identify cations and anions in a sample and analyze a solid, easily soluble sample. Detect the analyte in a sample, determine it by acid-base titration, calculate, prepare, and standardize a volumetric solution. Perform argentometric determination of sodium chloride in a liquid sample and potassium iodide and chloride in a solid sample. Differentiate analytes in a sample (Zn+Mg, Pb+Bi), determine them by chelatometric titration, and determine magnesium and calcium ions in hard water. Determine ascorbic acid in a Celaskon tablet and acetone in a liquid sample by iodometric titration. Standardize a volumetric solution of potassium permanganate and determine ferrous ions in a solid sample by manganometric titration. Determine hydroquinone by coulometric titration and calculate its content in a sample from the discharged electric charge. Determine nitrates and optionally fluorides or vice versa, using potentiometry with an ion-selective electrode, and construct a calibration curve. Perform gas chromatography to separate oxygen and nitrogen and evaluate the chromatogram. Determine acetylsalicylic acid in an Acylpyrin tablet by spectrophotometric method and construct a calibration curve. Extract ferric ions from mineral water into a solid phase (cation exchange resin) and determine the pre-concentrated analyte by spectrophotometry. During the course, the student will also likely: Use common sense. Calculate the weight of oxalic acid. Fill in several pages of a bound laboratory notebook. Place the cadmium ion solution on the correct shelf. Efficiently complete prescribed tasks. Evaluate the shape of calibration curves. Measure the potential of the silver electrode. Approximate the end of the practicum. Enjoy individual tasks. Explain inadequate preparation at home. Define their workspace on the lab table. Verify achieved results with supervisory staff. Assemble equipment for acid-base titration. Find the location of the semi-automatic burette. Compare reagents on the plate by sequence number. Assess the suitability of using a platinum wire for flame tests. Apply the skills gained in the practicum in their free time. Determine the optimal order of group and specific reagents. Distinguish between the orange-red and light violet coloring of the titration flask. Record the results of their observations in the laboratory notebook. Propose an optimal solution method. Recognize red precipitate from a blue soluble complex. Estimate the time for cleaning the workstation. Illustrate the course of redox titration. Explain the principles of analytical determinations. Seek help in case of an electric shock. Determine the minimum number of titration repetitions. Identify their workplace. Show appropriate laboratory footwear. Justify absence from the practical exercise. Find new friends. Attach a graphical output from the integrator to the protocol. Last update: Hraníček Jakub, RNDr., Ph.D. (23.09.2024)
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Instructions for Practical Tasks, Department of Analytical Chemistry, Charles University, 2004, the current version is available here in SIS as a separate file. CURRENT VERSION for WS 2024/2025: Version 20240927 Last update: Hraníček Jakub, RNDr., Ph.D. (23.09.2024)
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In order to obtain the credit from the basic course in analytical chemistry, students must successfully complete all practical tasks with an average grade of less than 2.5, pass the final written test and successfully carry out the final analysis of the practical sample.
Last update: Hraníček Jakub, RNDr., Ph.D. (23.09.2024)
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1. Qualitative analysis of inorganic cations and anions using wet chemistry: identification of two cations, identification of two anions Last update: Hraníček Jakub, RNDr., Ph.D. (23.09.2024)
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