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Course, academic year 2023/2024
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Advanced Laboratory Course - MC230C04
Title: Pokročilé praktikum z analytické chemie
Czech title: Pokročilé praktikum z analytické chemie
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2020 to 2023
Semester: winter
E-Credits: 10
Examination process: winter s.:
Hours per week, examination: winter s.:0/8, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: RNDr. Jana Sobotníková, Ph.D.
Teacher(s): RNDr. Hana Dejmková, Ph.D.
RNDr. Jan Fischer, Ph.D.
RNDr. Jakub Hraníček, Ph.D.
RNDr. Eliška Nováková, Ph.D.
prof. RNDr. František Opekar, CSc.
RNDr. Jana Sobotníková, Ph.D.
Annotation -
The following analytical methods are practiced in this course: electrochemical methods - anodic stripping voltammetry, conductometric titrations, and potentiometric titrations; separation methods - gas chromatography, high-performance liquid chromatography, and capillary zone electrophoresis; spectrometric methods - UV/VIS spectrophotometry, atomic absorption spectrometry and flow injection analysis with spectrophotometric detection.

The advanced laboratory course is scheduled 3 days a week, on Mondays, Wednesdays, and Fridays. It is necessary to attend the course all 3 days a week.

After completing the course, the student for these instrumental analytical methods (polarography, anodic stripping voltammetry, conductometry and conductometric titration, potentiometric titration, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):
- will explain the principle of the analytical methods listed above
- based on the instructions for the task, independently prepares solutions and uses instruments independently (performs measurements)
- interprets measured graphs and curves (polarographic and voltammetric curves, conductometric and potentiometric titration curves, absorption/emission spectra, electropherograms, chromatograms, calibration curves)
- describes and evaluates measured data, draws appropriate conclusions from them
- records the measurement procedure, results, and conclusions in a report
Last update: Sobotníková Jana, RNDr., Ph.D. (31.01.2024)
Literature -

1. Task Instructions and Safety Regulations can be downloaded from SIS.

2. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, 7th ed., 2017
3. D. C. Harris, Quantitative Chemical Analysis, 10th ed., 2019
4. D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch, Fundamentals of Analytical Chemistry, 10th ed., 2022

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Requirements to the exam -

This laboratory course concludes with an examination. The exam will be awarded upon successful completion of all prescribed tasks and submission of all protocols. The protocols must be submitted within the specified deadline, i.e. within 14 calendar days of the end of the laboratory course. The final grade of the examination is determined by the classification of the oral examination of each task and the classification of the submitted protocols, taking into account the continuous work of the student during the laboratory course.

Last update: Sobotníková Jana, RNDr., Ph.D. (29.05.2024)
Syllabus -

The Laboratory Course is scheduled 3 days a week, i.e. on Mondays, Wednesdays and Fridays.  The following practical tasks are realised:

1. Anodic stripping voltammetry: determination of Cd cation, optimization of experimental conditions, verification of method´s linearity, determination of limit of quantification, .

2. Conductometry: conductometric titration of various acids (strong, medium, weak), statistical evaluation of results, discussion of titration curves shapes.

3. Automatic potentiometric titration: optimization of conditions for titrimetric determination of divalent manganese by permanganate in the presence of sodium diphosphate.

4. Spectrophotometry: determination of active pharmaceutical compounds in a drug.

5. Atomic absorption spectrometry: determination of zinc in drinking water, optimization of experiemental conditions.

6. Flow injection analysis: inluence of basic experimental parameters on dispersion of the injected sample.

7. Gas chromatography: determination of methanol in fruit spirits.

8. High performace liquid chromatography: analysis of caffeine in beverages.

9. Capillary electrophoresis: analysis of inorganic anions in drinking water.

Last update: Sobotníková Jana, RNDr., Ph.D. (10.09.2021)
 
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