“Microscopic Techniques” is a course aimed to introduce students to various types of microscopy (light microscopy, fluorescence and electron microscopy) and the practical use of these techniques on model examples (counting of microscopic objects, digital image analysis). The course consists of 5 blocks. Active participation in all five parts of the course is necessary. For block 1, bring an A4 or unlined notebook, a pencil and a lab coat. The credit is given after the finalization of all the protocols in paper or electronic form. Teachers from different departments are involved in the teaching, and the individual blocks are spread throughout the semester. You will be informed about the time of the course by the guarantors of the individual blocks, to whom you can address your questions:
Block 1 and 2 and general questions about the course: Dr. Jovana Sádlová, sadlovaj@natur.cuni.cz.
Block 3: Dr. Veronika Sacherová, vsach@natur.cuni.cz
Block 4: Prof. Jana Albrechtová, jana.albrechtova@natur.cuni.cz.
Block 5: Dr. Aleš Benda, ales.benda@natur.cuni.cz
Looking forward to your participation,
Jovana Sadlova and colleagues
Last update: SADLOVA/NATUR.CUNI.CZ (26.08.2024)
„Mikroskopická technika“ je kurz, jehož cílem je seznámit studenty s různými typy mikroskopie (světelná mikroskopie, fluorescenční a elektronová mikroskopie) a s praktickým využitím těchto technik na modelových příkladech (počítání mikroskopických objektů, digitální analýza obrazu). Kurz se skládá z 5 bloků. Je nutná aktivní účast na všech pěti částech kurzu. Na 1. blok si přineste sešit formátu A4 nebo nelinkovaný sešit, tužku a laboratorní plášť. Zápočet se uděluje po dokončení všech protokolů v papírové nebo elektronické podobě. Na výuce se podílejí vyučující z různých kateder a jednotlivé bloky jsou rozloženy do celého semestru. O čase konání předmětu vás budou informovat garanti jednotlivých bloků, na které se můžete obracet se svými dotazy:
Dotazy k blokům 1 a 2 a obecné dotazy k předmětu: Jovana Sádlová, sadlovaj@natur.cuni.cz.
Blok 3: Veronika Sacherová, vsach@natur.cuni.cz.
Blok 4: Prof. Jana Albrechtová, jana.albrechtova@natur.cuni.cz.
Blok 5: Mgr: Mgr: Dr. Aleš Benda, ales.benda@natur.cuni.cz
Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Looking at the Structure of Cells in the Microscope. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26880/
A condition for obtaining credit is the completion of all five blocks and submission of a report (block 1) or completion of an electronic test (blocks 2-5).
Last update: SADLOVA/NATUR.CUNI.CZ (26.08.2024)
Syllabus
The course consists of five parts.
1. Light microscopy(Sádlová, Vojtková, Bečvář, Hammerbauerová, Jelínková, Vajs) Introduction to the basic theory of light microscopy and handling the light microscope. Students will prepare native and stained microscopic slides (Giemsa-Romanowski staining, aniline blue and native alizarin staining). The techniques will be demonstrated on protozoa with various taxonomic affiliation and helminths.
2. Special light and fluorescence microscopy(Sádlová, Vojtková, Bečvář, Hammerbauerová, Jelínková, Vajs) Students will be introduced into the basics of the theory of contrasts in the light microscopy (dark field, phase contrast, DIC, Hoffman contrast), fluorescence and confocal microscopy. They will practically learn the work with dark field, phase contrast and fluorescence microscope.
3. Electron microscopy (Sacherová, Juračka, Hyliš) Students will be introduced into the theory of electron microscopy and preparation of specimens. They will have chance to work practically with scanning and transmission electron microscope and prepare simple specimens.
4. Introduction to the image analysis (Albrechtová, Přibylová, Soukup et al.) In this section, students learn the basics of digitization and image processing and basic operations of image analysis. Practical exercises are conducted in the program ImageJ (National Institutes of Health, Bethesda, Maryland, USA, URL: http://imagej.nih.gov). More on the website of Block 4.
5. Advanced fluorescence and electron microscopy - excursion to the Service Laboratory of Imaging Methods BIOCEV (Aleš Benda, David Liebl, Dalibor Pánek, Piotr Jurkiewicz, Petra Prokšová, Marie Olšinová, Jiří Mikšátko, Daniela Popelková, Eva Ďurinová, Pelin Sunguret al.) Students will be introduced into assets and basic principles of advanced microscopical methods (Super-Resolution, Fluorescence Lifetime Imaging Microscopy (FLIM), Correlative Light Electron Microscopy) with excursion to the service laboratory and presentation of individual microscopes (STED microscope, STORM and SIM microscope, MP microscope, FLIM microscope, SEM microscope).
