SubjectsSubjects(version: 953)
Course, academic year 2023/2024
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Comparative Biochemistry of Parasites - laboratory course - MB160C17
Title: Praktikum z biochemie parazitů
Czech title: Praktikum z biochemie parazitů
Guaranteed by: Department of Parasitology (31-161)
Faculty: Faculty of Science
Actual: from 2023 to 2023
Semester: summer
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:0/1, C [TS]
Capacity: 12
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
the course is taught as cyclical
Guarantor: doc. RNDr. Ivan Hrdý, Ph.D.
Teacher(s): doc. RNDr. Ivan Hrdý, Ph.D.
Class: Horizontální laminární box 120 cm
Biohazard laminární box 200 cm
Stolní chlazená centrifuga univerzální se více rot
Invertovaný mikroskop s fluorescenční kamerou
Elektroforéza DNA horizontální
Zdroj napětí na elektroforézu
Pipetmany (sada)
Termoblok
Mikrocentrifuga stolní
Mikrocentrifuga malá
Dataprojektor
Mikroskop badatelský
Co-requisite : MB160P17
Annotation -
The course demonstrates basic techniques used for preparation of protist parasites for biochemical experiments. It demonstrates cell homogenization and fractionation using differential and isopycnic gradient centrifugation, spectrophotometric determinations of enzyme activities and liquid chromatography methods of protein purification.
Last update: Hrdý Ivan, doc. RNDr., Ph.D. (25.10.2019)
Literature -

Relevant literature: M.P. Deutscher: Guide to Protein Purification. Academic Press 1990.

 

Detailed and up-to-date protocols will available for every projet during the course.

Last update: Hrdý Ivan, doc. RNDr., Ph.D. (25.10.2019)
Requirements to the exam -

Active participation on the course, mastering of the methods, calculation of kinetic and molecular parameters of the studied proteins, 100% attendance.

Last update: Hrdý Ivan, doc. RNDr., Ph.D. (25.10.2019)
Syllabus -

1. Cell homogenization methods

2. Methods of cell fractionation, isolation of organelles

3. Principles of protein separation techniques

4. Enzyme activity determinations, kinetic measurements

5. Protein purification

Last update: Hrdý Ivan, doc. RNDr., Ph.D. (25.10.2019)
 
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