Molecular Immunology - MB150P15A
Title: Molekulární imunologie
Czech title: Molekulární imunologie
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2024 to 2024
Semester: winter
E-Credits: 5
Examination process: winter s.:
summer s.:combined
Hours per week, examination: winter s.:2/0, C [HT]
summer s.:2/0, Ex [HT]
Capacity: 25
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Explanation: Zimní semestr - individuální práce na projektuPřednášky probíhají v letním semestruKurz je organizován přes Moodle (e-learning) - odkaz vpravo
Note: enabled for web enrollment
priority enrollment if the course is part of the study plan
Guarantor: doc. Mgr. Jan Dobeš, Ph.D.
Teacher(s): prof. RNDr. Jan Černý, Ph.D.
doc. Mgr. Jan Dobeš, Ph.D.
Mgr. Ondřej Štěpánek, Ph.D.
Opinion survey results   Examination dates   WS schedule   SS schedule    E-learning course
Annotation -
The course is focused on deepening knowledge of the molecular mechanisms of selected immune processes, the basics of which were covered in the introductory Immunology lectures. In the winter semester, students work individually on an assigned project, while lectures take place in the summer semester.
Last update: Dobeš Jan, doc. Mgr., Ph.D. (25.09.2024)
Literature -

- Murphy K.: Janeway's Immunobiology (Immunobiology: The Immune System (Janeway)), 8th Edition, Garland Science Publishing 2011, 888 pages, ISBN-13: 978-0815342434
5. vydání je přístupné k vyhledávání na NCBI: http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=imm.TOC&depth=2
- A. K. Abbas, A. H. Lichtman, S. Pillai: Cellular and Molecular Immunology, 8th Edition, Saunders 2012, 560 pages, ISBN-13: 978-0323222754
http://books.google.cz/books?id=qtJY05rIUKQC
- T. J. Kindt, R. A. Goldsby, B. A. Osborne: Kuby Immunology, 7th Edition, W.H. Freeman, 2013, 574 pages, ISBN-13: 978-1429219198

Last update: Dobeš Jan, doc. Mgr., Ph.D. (25.09.2024)
Requirements to the exam -

Winter semester (zápočet):

To earn credits, students are expected to work continuously on a topic related to the Molecular Immunology course outline. They will prepare a presentation and talk, no longer than 5 minutes, covering a specific immunological molecular mechanism. Students select topics related to the course outline during the first week of the semester, with approval from the course guarantor.

By the end of November, students will present their work and receive feedback. Credits will be awarded upon submission of the final approved version of the presentation. This approved version will be presented by the students as part of the course during the summer semester.

 

In summer semester (exam):

During the summer semester, students can earn bonus points: an entrance test (1st lecture, max. 5 bonus points), a mid-term test (6th lecture, max. 10 bonus points), and active participation in lectures (max. 10 bonus points). At the end of the lectures in the summer semester, the exam will be conducted in the form of a written test (max. 100 points).

Grading:

95 or more points: Excellent (1)

85 or more points: Very good (2)

70 or more points: Good (3)

Less than 70 points: Failed (4)

Last update: Štěpánek Ondřej, Mgr., Ph.D. (02.02.2025)
Syllabus -

WINTER SEMESTER:
individual work on presentation related to the Molecular Immunology course outline, presentation of the results of individual wok.

SUMMER SEMESTER:

1. Introduction.
Models and experimental methods. Major signaling pathways – principles and overview. Entry test (max. 5 BONUS points).

2. How cells perceive infection?
Extracellular and intracellular receptors. Major innate signaling pathways. Danger signals. Interferons.

3. Major innate protective mechanisms.
Complement, phagocytosis and oxidative burst, degranulation, NETosis.

4. Receptors of adaptive immunity.
Generation of B- and T-cells repertoires. Antigen presentation. Cross-presentation. MHCI, MHCII, MHC-like molecules. NK receptors.

5. B-cell development and activation.
BCR signaling, isotypes, antibody-antigen recognition, in silico designed target binders

6. T-cell development and activation.
TCR signaling, TCR sequence – specificity relationship, TCR signaling, specific features of TCR, chimeric receptors. Mid-term test (max. 10 BONUS points)

7. Interplay between adaptive and innate immunity
NK cells, Fc receptors, type 1/2/3 immunity, ILCs, gamma-delta T cells

8. Co-stimulation and inhibitory signaling.
Costimulation and inhibitory receptors. Cytokine signaling, regulatory circuits, IL-2 as a model cytokine.

9. Cell death.
Types of cell death, pro-inflammatory and anti-inflammatory cell death, removal of dead cells, NK and T-cell mediated cell death, induced depletion of cells by antibodies, TNF-mediated cell death, interferons.

10. Immunometabolism.
Energy metabolism. Glycolytic switch. Role of metabolites in immune regulations. Polyamine synthesis.

11. Omics in immunology.
Systems immunology. Single cell RNAseq, spatial OMICs, proteomics.

 

Last update: Štěpánek Ondřej, Mgr., Ph.D. (16.03.2025)
Entry requirements -

The prerequisite is the completion and knowledge at the level of one of the basic immunology courses: Immunology (MB150P14E) or Imunologie (MB150P14B).

Last update: Dobeš Jan, doc. Mgr., Ph.D. (25.09.2024)