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Last update: RNDr. Gabriela Mikušová, Ph.D. (05.01.2018)
The course is held in Czech language. References: Brock Biology of Microorganisms, Eds. Madigan, Martinko, Stahl, Clark, 13th Edition, 2012, Pearson (or higher edition) Základy bakteriologie, F. Kaprálek, 1999, Karolinum |
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Last update: RNDr. Gabriela Mikušová, Ph.D. (05.01.2018)
Brock Biology of Microorganisms, Madigan, Martinko, Stahl, Clark (Eds.), 13th Edition, 2012, Pearson Education, Inc. |
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Last update: RNDr. Petra Lišková, Ph.D. (08.01.2024)
The exam is combined (written + oral part), questions are based on the sylabus. Topics are published in Moodle. |
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Last update: RNDr. Gabriela Mikušová, Ph.D. (25.10.2019)
1. Microorganisms and microbiology · properties of the cells, three domains of life, role of microorganisms in the environment · microbiology in historical context 2. Microbial cell structure and function · microscopy, Gram staining · cells of bacteria – cell wall, cytoplasmic membrane and transport, nucleoid, ribosomes, inclusions, flagellum, pili, capsule, gas vesicles, bacterial locomotion · endospores, sporulation 3. Molecular microbiology · bacterial genome - chromosome, plasmids · horizontal gene transfer - transformation, transduction, conjugation, transposition · gene cloning, mutation, selection · restriction endonucleases 4. Microbial metabolism · cell chemistry and nutrition · media and laboratory culture · energy classes of microorganisms · Bioenergetics – free energy, redox potential, activation energy, glycolysis, Krebs cycle, electron donors and acceptors, ATP · fermentation, respiration, anaerobic respiration, chemolithotrophy, phototrophy 5. Microbial growth · bacterial cell division – cell cycle, binary fission, peptidoglycan biosynthesis · population growth, growth curve, measuring microbial growth · continuous cultivation 6. Microorganisms and environment · effect of temperature on microbial growth, other environmental effects on microbial growth · control of microbial growth · microbial ecology, terrestrial Environments, aquatic Environments · symbioses between microorganisms, plants as microbial habitats, mammals as microbial habitats 7. Microbial evolution and systematics · early Earth and the origin and diversification of life, living fossils: DNA records, the history of life, microbial evolution, microbial systematics 8. Microbial Interactions with humans · Normal Human–Microbial Interactions · Pathogenesis, virulence factors, immunity, vaccination 9. Infectious diseases and their transmission · antimicrobial drugs · viruses · person-to-person bacterial and viral diseases, airborne bacterial and viral diseases, direct-contact diseases, sexually transmitted infections, vectorborne and soilborne infections, water and food as vehicles of bacterial diseases 10. Industrial microbiology and biotechnology · eukaryotic microbial cells · microorganisms in food industry · production of antibiotics, enzymes and vitamins, · bioremediation, wastewater and drinking water treatment |