SubjectsSubjects(version: 945)
Course, academic year 2023/2024
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Molecular Biology of RNA - MB140P44
Title: Molekulární biologie RNA
Czech title: Molekulární biologie RNA
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2022
Semester: winter
E-Credits: 3
Examination process: winter s.:oral
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: specialized
Explanation: změna názvu a semestru výuku od 2022/23
Note: enabled for web enrollment
Guarantor: RNDr. Tomáš Mašek, Ph.D.
Teacher(s): RNDr. Tomáš Mašek, Ph.D.
Annotation -
Last update: RNDr. Irena Lichá, CSc. (14.05.2009)
New series of lectures introduces students to selected topics in molecular biology and genetics. The first part of the course
consists of several advanced lectures concerning detailed mechanisms of eukaryotic translation, RNA metabolism and
control of gene expression on the posttrancriptional level. The rest of lectures - some of them held by external speakers - is
an overview of the most important findings in genomics, molecular biology of Eukaryotes and their viruses as well as new
significant techniques in the field. Course aims at teaching the biological and medicine science students at undergraduate
and graduate levels.
Literature -
Last update: RNDr. Tomáš Mašek, Ph.D. (12.05.2011)

Recommended literature:

Translational control in biology and medicine (Michael B. Mathews, Nahum Sonenberg, John W.B. Hershey, eds.), Cold Spring Harbor Monograph Series, Monograph 48, CSHL, 2007, ISBN 978-087969767-9

Recent issues of Nature, Science, PNAS, NAR and RNA journal.

Requirements to the exam -
Last update: RNDr. Tomáš Mašek, Ph.D. (22.12.2011)

Exam consists of two parts. First, the short test is needed to pass (6 questions, possibility of more correct answers, at least one correct answer, PhD students can skip this part of the exam). The second part has two questions usually covering broad range of subject matter, notes from lectures are allowed, not downloaded presentations).

Syllabus -
Last update: RNDr. Irena Lichá, CSc. (14.05.2009)

1. Structure of ribosome, initiation and elongation factors, translation initiation mechanism

2. Translation elongation and termination phases, eukaryotic versus prokaryotic translation, translation in plants

3. Overview of initiation site selection mechanisms, cap-dependent translation, IRES elements, re-initiation, ribosome shunting and hoping, programmed translational frameshifting, control of translation by upstream short ORF, termination codon read-through

4. Signaling to translation initiation and elongation, mTOR, TOP mRNAs, regulation of translation by amino acids, GCN4 paradigm, eIF2 kinases

5. Translational control during stress, heat shock response, interface of mRNA turnover and translation, RNA degradation, NMD, P-bodies, influence of polyadenylation on translation, translation through mRNA localization

6. Viral Translational Strategies and host defense mechanisms, Pikornaviruses, Reoviruses, Adenoviruses, influenza and Poxviruses

7. Translational control of developmental decisions, translation and miRNA, translation regulation in neurons, learning and memory, translational control in cancer development and during apoptosis, translational control in metabolic diseases, therapeutic opportunities in translation

8. Sequencing projects, comparative genomics and its applicability in current biology

9. SELEX and broad application of aptamers

10. 4E- family of initiation factors and control of gene expression

11. Cap structures and synthesis, capping enzymes, different types of mRNA 5´-ends in Eukaryotes, techniques to study capping

12. New advances in high-throughput technologies used in molecular biology, sequencing, microarrays, SAGE, qRT-PCR, etc.

13. Biology of Hepatitis C virus and related Flaviviruses, viral infection and host defense, searching for target for antiviral therapy

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