SubjectsSubjects(version: 945)
Course, academic year 2023/2024
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Molecular biology - MB140P41
Title: Molekulární biologie
Czech title: Molekulární biologie
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2020
Semester: winter
E-Credits: 5
Examination process: winter s.:combined
Hours per week, examination: winter s.:3/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: basic
Note: enabled for web enrollment
Guarantor: prof. RNDr. Zdena Palková, CSc.
Teacher(s): RNDr. Michal Čáp, Ph.D.
Mgr. Martin Kuthan, Ph.D.
prof. RNDr. Zdena Palková, CSc.
Is co-requisite for: MB140P82
Is complex co-requisite for: MB140P13, MB140P36, MB140P81
Annotation -
Last update: KONOP (29.04.2002)
The lectures follow the basic course of molecular biology and require the basic knowledge of processes of DNA replication, transcription and translation. The course is focused on principles and mechanisms of gene expression regulation in both prokaryotic and eukaryotic cells, including regulation of transcription, translation and protein stability. The molecular mechanisms of other cellular processes like protein sorting and distribution, membrane transport, cytoskelet function and basic processes of cell signalling are also emphasised. The course is recommended for students in 3rd year of the study.

Molecular Cell Biology (Lodish et al.), Molecular biology of the Cell (Alberts et al.), Molecular Biology of the Gene (Watson et al.),
Literature -
Last update: RNDr. Michal Čáp, Ph.D. (24.10.2019)


Molecular Cell Biology (Lodish et al.)

Molecular biology of the Cell (Alberts et al.)

Molecular Biology of the Gene (Watson et al.),

Requirements to the exam -
Last update: RNDr. Michal Čáp, Ph.D. (24.10.2019)

Written exam covers all the topics presented during the class.

Syllabus -
Last update: IRLICHA (12.05.2006)

Please note that the lectures are given in Czech language only.

1. Central dogma- repetition

Survey of basic molecular mechanisms: replication, transcription, translation and mRNA modifications (splicing).

2. Genome damage repair

Causes of genome DNA damage and basic types of processes participating in their repair.

3. Recombination and mobile genetic elements

Molecular mechanisms of recombination processes. Homologous and site specific recombination (examples). Transposable elements and their classification (DNA transposones and retrotransposones), mechanisms of transposition.

4. Gene expression regulation

Regulation of transcription in prokaryotic and eukaryotic cells (regulation of transcription initiation, atenuation). Regulation of mRNA modifications (alternative splicing, stability, editing, transport from nucleus to cytoplasm etc.). Regulation of translation initiation. Stability of proteins (degradation in proteasome, N-terminus rule, ubiquitine).

5. Transport processes inside the cell

Transport of proteins into different cell compartments. Secretion and endocytosis, role of endoplasmatic reticulum and Golgi apparatus, protein modifications and arrangements. Secretory vesicles (clathrin, COP vesicles). Transport into mitochondria, chloroplasts and peroxisomes.

6. Membranes and their functions

Transport through plasmatic membrane. Main types of transporters. Energy sources for single transport types.

7. Cytoskelet and its function

Actine and tubuline cytoskelet, its cell function. Role of motor proteins.

8. Principal pathways in cell signalization

Types of cell signalization. Main components (receptors, G-proteins, protein kinases, MAP kinases, small signal molecules etc.). Signalization from cell surface to nucleus.

9. New methods


Molecular Cell Biology (Lodish et al.), Molecular biology of the Cell (Alberts et al.), Molecular Biology of the Gene (Watson et al.),

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