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Last update: doc. RNDr. Radovan Fišer, Ph.D. (02.02.2021)
(a) on description of molecular structures of biopolymers (proteins and nucleic acids) and their subunits, including conformational polymorphism, polymorphism of sequences, special structures and topology of nucleic acids, (b) properties of biopolymers, which are important for their function, determination of their concentration, isolation, separation, sequencing, denaturation, reassociation, degradation, modification and analysis and (c) basic methods exploited for separation and analysis of biopolymers especially those which are important in connection with genetic engineering - techniques. The lecture is an extension of the lecture "Molecular biology" and complementary to "Genetic engineering". Moodle and PyMOL tools are used during the course. If the lessons take place online, the Zoom system will be used (the link will be sent to enrolled students by e-mail). |
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Last update: doc. RNDr. Radovan Fišer, Ph.D. (02.02.2021)
The course materials will be updated in the moodle environment https://dl2.cuni.cz/ Online lectures and exercises will take place using the Zoom system. Students will have recordings of lectures available.
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Last update: doc. RNDr. Radovan Fišer, Ph.D. (25.10.2019)
Please note, the lectures are given in Czech language only.
The exam takes the form of a test on the following topics:
1) Formulas, names and abbreviations of subunits and precursors of nucleic acids derived from major bases (+ pseudouridine, dideoxyNTP). Spatial arrangement and properties of these structures, keto-enol, amino-imino tautomers, atom numbering and torsion angle marking, base analogs, mutability, hydration, hydrogen bridges, Watson-Crick base pairs. 2) Coded amino acids-names, three-letter abbreviations, dissociation and association patterns, approximate values of pKa groups and their names, acidic, basic neutral amino acids, polar and non-polar, aromatic, containing S, -OH and various other groups. Chirality, hydrogen bridges and hydration, peptide and isopeptide bond- mesomerism. 3) Basic concepts, formulas and small tasks to solve around the structure and properties of biopolymers. 4) Small tasks about using and applying methods for analysis and separation of biopolymers. A total of 3 optional tests are written during the exercises, the results of which are reflected in the final grade. There are no substitute dates for ongoing tests. The resulting mark "excellent" can be achieved even without continuous tests. The tutorial is supplemented by tasks assigned in the Moodle environment. Solving these tasks is not necessary, but is appropriate. Some exam questions will follow up on the Moodle tasks. The result will show the following results: A = 1st optional test -> max. 7 points B = 2nd optional test -> max. 7 points C = 3rd optional test -> max. 7 points Z = Test result -> 0 - 100 points If Z is> 60, then: The sum of points X = A + B + C + Z If Z <60, then: The sum of points X = Z Marking: X> 90 => grade 1 X ∊ (75-90) => grade 2 X ∊ (60-75) => grade 3 X <60 => failed This means that who does not pass the test will not help him / her. Others may improve grades due to optional tests. |
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Last update: doc. RNDr. Radovan Fišer, Ph.D. (02.02.2021)
Please note, the lectures are given in Czech language only. The lecture will be recorded by the teacher (sound + projected image). These materials will be accessible online. Details of quantitative polymerase chain reaction (Real-time PCR or qPCR) |