SubjectsSubjects(version: 953)
Course, academic year 2023/2024
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Microbiology - MB140P33
Title: Mikrobiologie
Czech title: Mikrobiologie
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2023
Semester: winter
E-Credits: 6
Examination process: winter s.:written
Hours per week, examination: winter s.:3/0, C+Ex [HT]
Extent per academic year: 3 [days]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: basic
Note: enabled for web enrollment
Guarantor: RNDr. Gabriela Mikušová, Ph.D.
Teacher(s): RNDr. Ondřej Černý, Ph.D.
RNDr. Tereza Dolejšová, Ph.D.
RNDr. Petra Lišková, Ph.D.
RNDr. Gabriela Mikušová, Ph.D.
RNDr. Jaroslav Nunvář, Ph.D.
Mgr. Irena Vopálenská, Ph.D.
Pre-requisite : {At least one of the following subjects: MB150P04A, MB150P04B, MB150P34, MC250P03C.}
Incompatibility : MB140P33I
Is incompatible with: MB140P26, MB140C88, MB140P88
Is pre-requisite for: MB140P70, MB140P34
In complex pre-requisite: MB140C13
Annotation -
The lecture is intended for students of the bachelor's degree in biology.

The condition of the microbiology exam is a credit from the laboratory course for the lecture.

The lecture presupposes previous completion of lecture: Biochemistry (see "Prerequisites"). We also recommend attending lectures: Cell Biology, Molecular Biology.

Contact email:
Last update: Mikušová Gabriela, RNDr., Ph.D. (18.09.2023)

Madigan, Martinko, Berger, Buckley, Stahl: Brock Biology of Microorganisms, Pearson; 15 edition (2017), ISBN-10: 9780134261928

Madigan, Bender, Buckley, Sattley, Stahl: Brock Biology of Microorganisms, Pearson, Global Edition, 16th edition (2021), ISBN: 9781292404950

Last update: Mikušová Gabriela, RNDr., Ph.D. (18.09.2023)
Requirements to the exam -

Students will be evaluated on the basis of a written test. Source: lectures, presentations, compulsory literature (Brock Biology of Microorganisms)

It is possible to enrol for the examination only after completing the credit from practical exercises.

Last update: Mikušová Gabriela, RNDr., Ph.D. (18.09.2023)
Syllabus -
1. Microorganisms and microbiology.
Introduction, microorganisms, milestones of microbiology, observation of microorganisms, special properties of microorganisms, viruses.

2. Structure and function of bacterial cell.
Cell shape, size, cytoplasmic membrane, transport mechanisms, cell wall, other cell structures, mechanisms of motion.

3. Nutrition and metabolism of microorganisms.
Nutrition and composition of microorganisms, microbiological media, electron transporters, ATP, Glycolysis, Krebs cycle, aerobic respiration.

4. Growth of microorganisms
Individual cell growth, Exponential growth and growth curve phase. Temperature, pH, osmotic pressure and other physical factors affecting growth.

5. Molecular biology and genetics of bacteria and archea.
Escherichia coli chromosome, plasmids, DNA replication, transcription and sigma factors, horizontal transfer of genetic information - transformation, transduction and conjugation. Mobile DNA. Bacterial genomics.

6. Diversity of metabolisms. Industrial microbiology and biotechnology.
Phototrophy and chemolitotrophy, fermentation and anaerobic respiration. Products of microorganisms in industrial microbiology. Products of genetically modified microorganisms.

7. Evolution of microorganisms and their systematics.
Earth evolution, origin and diversification of life. Phylogeny of microorganisms. The concept of species, classification and nomenclature.

8. Diversity of bacteria.
Phototrophic bacteria, pseudomonads, enterobacteria, Vibrio and Photobacterium, morphologically characteristic proteobacteria, mycobacteria, staphylococci and streptococci, endospore-forming bacteria, mycoplasmas, streptomyces, cyanobacteria, green sulfur bacteria, spirochetes. Archea, evolution and life in high temperature.

9. Inhibition of growth of microorganisms.
Suppression of microorganisms growth by physical factors and chemical substances.

10. Microorganisms and man.
Beneficial relationships of the host with microorganisms. Virulence factors and pathogenicity of bacteria, infection and host response. Examples: airborne diseases, sexually transmitted diseases, animal vectors, food.
Last update: Konopásek Ivo, doc. RNDr., CSc. (25.10.2019)
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