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Course, academic year 2023/2024
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Molecular biology for the students of biology education programmes - MB140P21
Title: Základy molekulární biologie pro učitele
Czech title: Základy molekulární biologie pro učitele
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2022 to 2023
Semester: summer
E-Credits: 3
Examination process: summer s.:combined
Hours per week, examination: summer s.:3/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: basic
Is provided by: MB140P20
Note: enabled for web enrollment
Guarantor: Mgr. Martin Kuthan, Ph.D.
Teacher(s): Mgr. Martin Kuthan, Ph.D.
Class: Ultracentrifuga a 3 rotory
Gradientový PCR cycler
Incompatibility : MB140P20
Is complex co-requisite for: MB140C20, MB140P13, MB140P36, MB140P81
Annotation -
The lecture is focused on the basic mechanisms and principles of molecular biology with regard to the needs of students in teacher education. The main part of the lecture is devoted to the storage and copying of genetic information in the cell and to the expression and regulation of genes. In addition, the lecture includes an introduction to basic methods of molecular biology.
Last update: Kuthan Martin, Mgr., Ph.D. (30.10.2019)
Literature -

Alberts et al., Základy buněčné biologie: Úvod do molekulární biologie buňky. Espero Publishing, 2005 (český překlad)
Alberts et al., Molecular biology of the cell, Garland Science, 2002
Lodish et al., Molecular cell biology, Freeman 2000
Watson et al., Molecular Biology of the Gene, Pearson, 2004
Watson et al., Rekombinantní DNA : krátký kurs, Academia, 1988

Last update: Kuthan Martin, Mgr., Ph.D. (30.10.2019)
Requirements to the exam -

Zkouška je písemná a obsahově odpovídá přednášené látce a materiálům, které jsou studentům k dispozici. Písemný test je složen z několika typů otázek (vysvětlení pojmů, multiple choice a volné odpovědi na zadané otázky).

Last update: Kuthan Martin, Mgr., Ph.D. (30.10.2019)
Syllabus -
Structure and function of biomacromolecules
building blocks and chemical bonds in DNA, RNA and proteins, the importance of weak interactions for the structure of macromolecules, the importance of energy-rich compounds

Genetic information
molecular basis of heredity, current concept of genes, structure and function of DNA and RNA. Information flow in biological systems? central dogma of molecular biology

Chromosomes in cells
chromosomal DNA, structure and function of chromosomes, chromatin and nucleosomes, centromere and telomeric functions, organelle genomes

Copying genetic information
DNA replication, chromosome and extra-chromosomal DNA replication, linear chromosome end replication

Recombination and mobile genetic elements
role of recombination homologies, genetic consequences of recombination processes, mechanisms of homologous and site-specific recombination, mobile DNA? transposons, retroposons, retroviruses, biological role of site-specific recombination

Mutation and repair of damaged DNA
physiological and non-physiological modifications of DNA, mutations - causes and consequences of DNA damage, mechanism of repair processes, possibilities of repairing damaged DNA and repairing errors caused by DNA replication

Manifestation of genetic information
mechanisms of transcription in prokaryotes and eukaryotes, RNA processing, structure and function of tRNA

RNA splicing
splicing mechanisms, ribozymes, RNA editing, RNA world

Protein synthesis
prokaryotic and eukaryotic translation, genetic code, chaperones and tertiary structure of proteins, posttranslational modification of proteins and role of endoplasmic reticulum and Golgi apparatus, protein degradation

Regulation of genes
basic principles of gene expression regulation, transcription activators and repressors, regulation of prokaryotic and eukaryotic initiation of transcription, attenuation, regulation of mRNA modifications, changes in chromatin structure and gene silencing, regulation of translation initiation, regulation of protein stability, regulation of genes important for development

Application of molecular biology and genetic engineering techniques
DNA cloning, DNA sequencing and genomic projects, genetically modified organisms, genetic tests, gene therapy
Last update: Kuthan Martin, Mgr., Ph.D. (30.10.2019)
 
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