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Course, academic year 2023/2024
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Practical course on microbiology for the students of Biology Education programmes - MB140C88
Title: Praktikum z mikrobiologie pro učitele
Czech title: Praktikum z mikrobiologie pro učitele
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2023 to 2023
Semester: summer
E-Credits: 1
Examination process: summer s.:
Hours per week, examination: summer s.:0/2, C [DS]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: basic
Explanation: Praktikum se v termínu uvedeném v rozvrhu bude konat.
Note: enabled for web enrollment
Guarantor: RNDr. Tereza Dolejšová, Ph.D.
Teacher(s): RNDr. Tereza Dolejšová, Ph.D.
RNDr. Petra Lišková, Ph.D.
Mgr. Irena Vopálenská, Ph.D.
RNDr. Blanka Zikánová
Co-requisite : MB140P88
Incompatibility : MB140P33, MB140P33I
In complex pre-requisite: MB140C13
Requirements to the exam

Credit is awarded for active participation and submission of task reports for review.

Last update: Lišková Petra, RNDr., Ph.D. (25.10.2019)
Syllabus

1 / Preparation of culture media and sterilization.
Types and composition of culture media, sterilization methods, preparation of sterile glass and instruments, disposal of used experimental material.
2 / Vaccination and aseptic work.
Aseptic techniques, work with pure culture, isolation of microorganisms, enrichment techniques, cultivation conditions.
3 / Native and permanent preparations, Gram staining of bacteria, staining of spores.
4 / Quantitative tasks with microorganisms.
Determination of number of live bacteria in acidophilic milk by sowing on solid culture medium, monitoring of presence of coliform bacteria in water samples. Determination of the total number of microorganisms, counting chamber.
5 / Approaches to identification of bacteria. Monitoring of biochemically important properties of bacteria, identification of unknown enterobacteria by commercial test (Enterotest).
6 / Influence of environmental factors (pH) on the growth of microorganisms, determination of optimal pH for selected strains of bacteria and yeasts.

Last update: Lišková Petra, RNDr., Ph.D. (25.10.2019)
 
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