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Course, academic year 2023/2024
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Molecular biology and virology practical training course - MB140C77
Title: Praktikum z molekulární biologie a virologie
Czech title: Praktikum z molekulární biologie a virologie
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2022
Semester: summer
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:0/1, C [TS]
Capacity: 18
Min. number of students: 5
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: specialized
Note: enabled for web enrollment
Guarantor: Mgr. Vojtěch Šroller, Ph.D.
Teacher(s): Mgr. Martin Fraiberk, Ph.D.
RNDr. Lenka Horníková, Ph.D.
Boris Rjabčenko, Ph.D.
Mgr. Vojtěch Šroller, Ph.D.
Class: Horizontální laminární box 120 cm
Biohazard laminární box 200 cm
Stolní chlazená centrifuga univerzální se více rot
Invertovaný mikroskop s fluorescenční kamerou
Elektroforéza DNA horizontální
Zdroj napětí na elektroforézu
Pipetmany (sada)
Termoblok
Mikrocentrifuga stolní
Mikrocentrifuga malá
Dataprojektor
Mikroskop badatelský
Annotation -
One week course from molecular biology and virology provides both practical and theoretical training in laboratory
methods from molecular biology and virology. The selected experiments allow students to practice not only basic
methods (like DNA isolation, gel electrophoresis) but also methods more advances, which are used in biomedical
research and diagnostics laboratories (like electron microscopy, PCR, sequencing). Laboratory course is well
suited for students interested in molecular biology and virology.
Last update: Šroller Vojtěch, Mgr., Ph.D. (08.01.2024)
Literature -

Brown T.A. (2006, 2010) Gene Cloning and DNA Analysis: An Introduction; Willey-Blackwell

Sambrook J., Russell D.W., (2001) Molecular Cloning: A Laboratory Manual, third edition, Cold Spring Harbor Laboratory Press

Vondrejs V. (1997, 2001, 2003), Genové inženýrství I-III, Karolinum

Last update: Šroller Vojtěch, Mgr., Ph.D. (25.10.2019)
Requirements to the exam -

credit based on written protocols

Last update: Šroller Vojtěch, Mgr., Ph.D. (30.08.2019)
Syllabus -

The work schedule of practical course:

1. Isolation of DNA, RNA and proteins from mouse cell line, measuring concentration and purity (spectrophotometry)

2. Isolation of bacterial plasmid DNA, visualization on gel electrophoresis, restriction analysis, methods for cloning of genes.

3. Detection of virus specific antibodies in pigs’ sera, using hemagglutination and neutralization inhibition test.

4. Visualization of pseudovirions by electron microscopy.

5. Isolation and detection viral DNA from skin using methods rolling cycle amplification, PCR multiplex amplification, sequencing and identification of DNA by computer analysis.

Last update: Lichá Irena, RNDr., CSc. (26.05.2017)
 
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