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Course, academic year 2023/2024
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Practical courses in virology - MB140C74
Title: Cvičení z virologie
Czech title: Cvičení z virologie
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 1
Examination process: summer s.:
Hours per week, examination: summer s.:0/1, C [DS]
Capacity: 96
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: basic
Explanation: jednodenní turnusové praktikum
Note: enabled for web enrollment
Guarantor: Mgr. Martin Fraiberk, Ph.D.
Teacher(s): Mgr. Kateřina Bruštíková, Ph.D.
Mgr. Martin Fraiberk, Ph.D.
RNDr. Lenka Horníková, Ph.D.
Mgr. Vojtěch Šroller, Ph.D.
Class: Horizontální laminární box 120 cm
Biohazard laminární box 200 cm
Stolní chlazená centrifuga univerzální se více rot
Invertovaný mikroskop s fluorescenční kamerou
SEM s vysokým rozlišením
Elektroforéza DNA horizontální
Zdroj napětí na elektroforézu
Pipetmany (sada)
Termoblok
Mikrocentrifuga stolní
Mikrocentrifuga malá
Dataprojektor
Mikroskop badatelský
Co-requisite : {At least one of the following subjects: MB140P75, MB140P75U}
Annotation -
The course is an optional extension of the basic lecture course "Fundamentals of Virology" MB140P75. Based on clinical examples, students will practice the subject matter and learn more. During the exercise students will also be able to look behind the scenes of the virology laboratory and try some basic laboratory approaches used in virology. The course will also provide information on the basics of work in the laboratory with a biosafety level of protection 2, which also allows work with human pathogens. Course participants will be able to test such work in a non-pathogenic virus - mouse polyomavirus and BK virus.
Last update: Fraiberk Martin, Mgr., Ph.D. (21.02.2023)
Literature -

Cann, A. Principles of Molecular virology 4th edition, Elsevier, 2005
for advanced readers: Knipe, Howley, Fields Virology 5th edition

Last update: Schierová Michaela, RNDr., Ph.D. (24.10.2019)
Requirements to the exam -

The course-unit credit is awarded for participation, completion of the task and completion of protocols. PROTOCOLS ARE SUBMITTED BY EVERY PRACTICAL PARTICIPANT

Last update: Schierová Michaela, RNDr., Ph.D. (24.10.2019)
Syllabus -

The lectures are given in Czech language only.

During the one-day exercise, all students will try to determine the number of viral particles by hemagglutination, PCR determination of viral DNA, immunodetection on the membrane

Last update: Fraiberk Martin, Mgr., Ph.D. (21.02.2023)
Entry requirements -

TO WORK IN course lab, IT IS NECESSARY TO HAVE laboratory clothes and shoes WITH YOU;

Last update: Schierová Michaela, RNDr., Ph.D. (24.10.2019)
 
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