SubjectsSubjects(version: 953)
Course, academic year 2023/2024
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Practical course of diagnostic methods in virology - MB140C69E
Title: Practical course of diagnostic methods in virology
Czech title: Praktický kurz diagnostických metod ve virologii
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2023 to 2023
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:0/1, C [TS]
Capacity: 12
Min. number of students: 6
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: English
Note: enabled for web enrollment
Guarantor: RNDr. Ingrid Poláková, Ph.D.
Teacher(s): RNDr. Ingrid Poláková, Ph.D.
RNDr. Martina Saláková, Ph.D.
RNDr. Jana Šmahelová
Class: Biohazard laminární box 200 cm
Stolní chlazená centrifuga univerzální se více rot
Invertovaný mikroskop s fluorescenční kamerou
Elektroforéza DNA horizontální
Zdroj napětí na elektroforézu
Pipetmany (sada)
Termoblok
Mikrocentrifuga stolní
Mikrocentrifuga malá
Dataprojektor
Původní předmět
Incompatibility : MB140C85
Is incompatible with: MB140C85
Annotation -
This practical course will focus on molecular methods of viral detection in clinical specimens including the
interpretation of results. The methods will include extraction of nucleic acids from different type of clinical material. The students will analyze the quality and quantity of nucleic acids. They will also detect the selected genes by the polymerase
chain reaction (PCR) and/or real time PCR. The specificity of amplicons will be confirmed by sequencing.
For the detection of viral specific antibodies Enzyme-Linked Immunosorbent
Assay (ELISA) and/or indirect immunofluorescence method will be used.
The course will be held in BIOCEV, Vestec from Oct 9 to 13.
Last update: Poláková Ingrid, RNDr., Ph.D. (22.08.2023)
Literature -

Fields Virology, Fields, Knipe and Howley, 2013, 6th edition

Last update: Poláková Ingrid, RNDr., Ph.D. (13.12.2021)
Requirements to the exam -

 The course will be held in BIOCEV, Vestec from Oct 9 to 13

In order to obtain credits the students have to provide the protocols per each group (2 students per group). The protocols have to include interpretation of the results. 

Last update: Poláková Ingrid, RNDr., Ph.D. (22.08.2023)
Entry requirements -

ATTENTION! To pass an entrance exam is a requirement (basics knowledge in molecular biology an virology).
The entrance exam will be held the first week in October. The exact date and place will be sent by email.

Last update: Poláková Ingrid, RNDr., Ph.D. (22.08.2023)
 
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