Genetics of procaryot - practical lectures - MB140C31

• Title: Genetika prokaryot - cvičení
• Czech title: Genetika prokaryot - cvičení
• Guaranteed by: Department of Genetics and Microbiology (31-140)
• Faculty: Faculty of Science
• Actual: from 2023
• Semester: summer
• E-Credits: 1
• Examination process: summer s.:
• Hours per week, examination: summer s.:0/1, C [HT]
• Capacity: 24
• Min. number of students: 5
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: cancelled
• Language: Czech
• Explanation: dodatečně nevyučován (onemocnění garantky)
• Note: enabled for web enrollment
• Guarantor: RNDr. Irena Lichá, CSc.
• Co-requisite : MB140P13

Annotation

English

Last update: RNDr. Irena Lichá, CSc. (22.03.2019)

Exercise should complement lectured topics in the course Genetics of prokaryotes and practice of specific examples.

Czech

Last update: RNDr. Irena Lichá, CSc. (07.11.2022)

kurz v letním semestru 22/23 nebude vyučován pro zdravotní problémy přednášejícího


Cvičení by mělo doplnit odpřednášená témata v kurzu Genetika prokaryot a procvičit je na konkrétních příkladech.

Literature

English

Last update: RNDr. Irena Lichá, CSc. (27.10.2019)

Molecular Genetics of Bacteria, Snyder, L., and Champness, W. 2nd Ed.

Comprehensive articles from www.ELS. NET, listed on the lecturer's website for the lecture. The course is also available for registered students on Moodle.

Czech

Last update: RNDr. Irena Lichá, CSc. (27.10.2019)

Molecular Genetics of Bacteria, Snyder, L. and Champness, W. 2nd Ed., (ASM Press), 2003. a 3th Ed (ASM press 2007).

Souborné články z portálu www.ELS. NET, uvedené na stránkách vyučujícího k dané přednášce. Kurs je téžpro přihlášené studenty dostupný na Moodle.

Requirements to the exam

English

Last update: RNDr. Irena Lichá, CSc. (27.10.2019)

Credit is awarded both for active participation in exercises during the semester, two absences are tolerated. In addition, during the course, four tasks are assigned for individual elaboration. The evaluation takes place via the Moodle portal.

Czech

Last update: RNDr. Irena Lichá, CSc. (27.10.2019)

Zápočet se uděluje jednak za aktivní účast na cvičení během semestru, tolerují se dvě absence. Dále jsou během kurzu zadány čtyři úlohy k samostatnému vypracování. Vyhodnocování probíhá přes portál Moodle.

Syllabus

Last update: RNDr. Irena Lichá, CSc. (27.10.2019)

1. Genome structure and its analysis. Searching in databases of genomes of bacteria and archaea, structure of genes, searching for information about gene properties, their sequences.

2. Structure of transposons and IS elements. Properties of transposases - search using BLAST, specialized database for transposons (IS finder).

3. Properties of plasmids. Genetic evidence of plasmid replication mechanism, RNAI inhibition in CoEl1 plasmid. Study of plasmid compatibility, genetic versus bioinformatic approach.

4. Bacteria and archaea viruses. Sources of information on the phages of bacteria and archaea –Viralzone. Molecular biology of viruses - replication, integration, host-virus interaction. Examples of bacteriophages use in genetics, multiplicity of infection, phage titer determination. Use of vectors derived from phage lambda in metagenomics.

5. Genetic versus physical mapping. Conjugative mapping - examples. Non-specific transduction, principles of transduction mapping and use in mutant preparation - examples. Three-factor crosses examples.

6. Basics of genetic analysis. Examples of mutant evaluation, principles of mutant selection, selection conditions, reverse versus suppressor mutation, genetic evidence of intra / inter gene suppressor mutation. Mutation localization in gene, marker rescue, deletion mapping, examples.

7. Principles of non-specific mutagenesis. Calculation of mutation rate. Transposon mutagenesis - an example of the Nebraska transposon library. 

8. Targeted mutagenesis. Allele exchanges, examples of strategies in different bacteria. Selection strategies for single versus double recombinants.