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Course, academic year 2024/2025
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Examination methods in microbiological laboratory - GF374
Title: Vyšetřovací metody v mikrobiologické laboratoři
Guaranteed by: Department of Biological and Medical Sciences (16-16150)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2024
Semester: summer
Points: 0
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:0/42, C [HS]
Capacity: unknown / unlimited (40)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Note: deregister from the credit exam date if a requisite was not fulfilled
course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: PharmDr. Ondřej Janďourek, Ph.D.
Comes under: 3.ročník 2024/25 Farmacie
Incompatibility : GF367
Pre-requisite : GF366, GF371
Interchangeability : GF367
Annotation -
Subject Examination methods in microbiological laboratory is following the subjects Microbiology and Practical classes from microbiology with the aim to extend and deepen primarily knowledge of methodical approach used in laboratories of clinical microbiology as well as pharmaceutical microbiology or food microbiology. The subject is focused on microbiological diagnostics of infectious agents and infectious diseases. It means especially knowledge of routine laboratory and clinical diagnostics together with actual modern trends and methods. Student should manage microbiological issues in range necessary for work in microbiological laboratories.
Last update: Janďourek Ondřej, PharmDr., Ph.D. (18.07.2024)
Course completion requirements -

Credit conditions:

  • 100% attendance in laboratory practice
  • Worked out protocols from each lecture handed in not later than given date
  • Average grade from protocols have to be 2.0 or better
  • Successful accomplished practical exam
  • Successfully passed credit test

 

Last update: Janďourek Ondřej, PharmDr., Ph.D. (18.07.2024)
Literature - Czech

Povinná:

  • . . In Ryšková, Olga. Praktická cvičení a semináře z lékařské mikrobiologie pro studující všeobecného a zubního lékařství . Praha: Karolinum, 2010, s. -. ISBN 978-80-246-1834-0..

  • . . In Votava, Miroslav. Lékařská mikrobiologie . Brno: Masarykova univerzita, 2000, s. -. ISBN 80-210-2272-8..

  • . . In Votava, Miroslav. Lékařská mikrobiologie obecná . Brno: Neptun, 2005, s. -. ISBN 80-86850-00-5..

  • . . In Votava, Miroslav. Lékařská mikrobiologie speciální . Brno: Neptun, 2003, s. -. ISBN 80-902896-6-5..

  • . . In Votava, Miroslav. Kultivační půdy v lékařské mikrobiologii . Brno: Nakladatelství Hortus, 2000, s. -. ISBN 80-238-5058-X..

  • . . In Votava, Miroslav. Lékařská mikrobiologie - vyšetřovací metody . Brno: Neptun, 2010, s. -. ISBN 978-80-86850-04-7..

Doporučená:

  • . . In Kruif, Paul de. Lovci mikrobů . Praha: Orbis - Mladá fronta, 1959, s. -. ISBN ..

  • Kolářová, Libuše. Obecná a klinická mikrobiologie. : Galén, 2020, 441 s. ISBN 978-80-7492-477-4.

  • Hurych, Jakub, Štícha, Roman. Repetitorium lékařské mikrobiologie. Praha: Stanislav Juhaňák - Triton, 2021, 624 s. ISBN 978-80-7553-900-7.

  • Scharfen, Josef. Diferenciální diagnostika v klinické mikrobiologii. Praha: RNDr. František Skopec, CSc. - Nucleus HK, 2013, 223 s. ISBN 978-80-87009-32-1.

Last update: prepocet_literatura.php (19.09.2024)
Teaching methods -
  • Seminars – PPT presentations, discussion, theory for practical tasks
  • Practical classes – identification methods used in microbiological laboratory, microbiological techniques and evaluation of results
Last update: Janďourek Ondřej, PharmDr., Ph.D. (18.07.2024)
Requirements to the exam -
Knowledge of subject stated by supports mentioned in syllabus
Last update: Janďourek Ondřej, PharmDr., Ph.D. (18.07.2024)
Syllabus -
  • Harvesting and processing of microbiological material, microbiological laboratory operation
  • Cultivation media, cultivation proof of bacteria
  • Laboratory diagnostics of Gram negative rods
  • Laboratory diagnostics of Gram positive cocci. Haemocultures, sterility controls.
  • Laboratory diagnostics of fastidious pathogens
  • Laboratory diagnostics of Gram positive rods
  • Laboratory diagnostics of acid-fast bacteria
  • Laboratory diagnostics of parasite infections
  • Laboratory diagnostics of viral infections
  • Laboratory diagnostics in mycological laboratory
  • Indirect laboratory diagnostics for infectious diseases  - serological methods and direct proof of antigens
  • Pharmaceutical microbiology methods – smear methods, determination of microbial contamination of pharmaceutical products, …
  • Instrumental methods in microbiological laboratories
Last update: Janďourek Ondřej, PharmDr., Ph.D. (18.07.2024)
Learning resources -

https://intranet.faf.cuni.cz/Studijni-materialy/KBLV/?path=l%c3%a9ka%c5%99sk%c3%a1+mikrobiologie%5cp%c5%99edn%c3%a1%c5%a1ky+l%c3%a9ka%c5%99sk%c3%a1+mikrobiologie

https://intranet.faf.cuni.cz/Studijni-materialy/KBLV/?path=l%c3%a9ka%c5%99sk%c3%a1+mikrobiologie%5ccvi%c4%8deni+lm+2017

https://intranet.faf.cuni.cz/Studijni-materialy/KBLV/?path=vy%c5%a1et%c5%99ovac%c3%ad+metody+v+mikrobiologick%c3%a9+laborato%c5%99i

Last update: Janďourek Ondřej, PharmDr., Ph.D. (18.07.2024)
Learning outcomes -

The course Examination Methods in Microbiological Laboratory builds on the knowledge and skills acquired in the courses: Microbiology and Practical Classes in Microbiology.

At the end of the course, students will be able to use the following terms (including their commonly used abbreviations) in the correct context towards the workflows used in clinical microbiological laboratories and to evaluate the results obtained: sterilization, disinfection, microscopic techniques, temporary and permanent mounts, staining techniques in microbiology (Gram, Ziehl-Neelsen), MIC, MBC, disc diffusion test, dilution methods, EUCAST, CLSI, biochemical identification, types of cultivation media,  pharmaceutical microbiology, microbiological safety, Czech Pharmacopoeia (ČL).

Learning outcomes:

On the basis of the knowledge and skills acquired, students will:

Ø  define the importance of the microbiological laboratory in relation to the diagnosis of infectious diseases;

Ø  they are familiar with basic microbiological techniques and procedures;

Ø  they are familiar with and know how to use common microbiological standards (EUCAST, CLSI)

Ø  choose a suitable technique and prepare different types of microscope slides based on the type of sample;

Ø  perform appropriate methods for identifying the unknown agent;

Ø  perform susceptibility determination of the microbial agent and interpret the results against standards

Ø  evaluate and interpret the obtained experimental data in connection with standards or pharmacopoeia requirements in the determination of microbial safety;

Ø  propose an appropriate procedure for identifying and determining susceptibility based on preliminary sample information

Last update: Janďourek Ondřej, PharmDr., Ph.D. (31.03.2025)
 
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