Analytical Chemistry - GF300
Title: Analytická chemie
Guaranteed by: Department of Analytical Chemistry (16-16140)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2023 to 2025
Semester: winter
Points: 0
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:14/42, C [HS]
Capacity: unlimited / 330 (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Note: deregister from the credit exam date if a requisite was not fulfilled
course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: doc. PharmDr. Hana Sklenářová, Ph.D.
Comes under: 2.ročník 2025/26 Farmacie
Pre-requisite : GF299
Interchangeability : GF139
Is co-requisite for: GF301, GF302, GF395
Is interchangeable with: GF139
WS schedule   
Annotation -
The aim of the subject is to provide the students with firm and sufficiently wide theoretical and methodological fundamentals for pursuing (in a rational way) chemical analysis of inorganic and organic substances, and simple approaches in volumetric analysis based on titrations of different types. The teaching is focused on the explanation of theoretical aspects of instrumental methods. In practical training individual approach of students to solving analytical problems is required.
Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (22.08.2025)
Course completion requirements -

REQUIREMENTS FOR CREDIT

Practical course

  1. Participation in all practical exercises - possibility of compensation after agreement with the teacher
  2. Compliance with safety rules when working in the laboratory
  3. Submission of reports of all practical exercises
  4. Achieving the minimum number of points in the practical part and the theoretical part (continuous tests and credit test) - 2/3 of the total number
  5. In the case of 3 unsuccessful attempts at a continuous or credit test - examination of knowledge before a 2-member committee
  6. Replacement of possibly broken measuring glass
Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (22.08.2025)
Literature - Czech

Povinná:

  • Karlíček, Rolf. Analytická chemie pro farmaceuty. Praha: Karolinum, 2013, 281 s. ISBN 978-80-246-2202-6.

  • Karlíček, Rolf. Analytická chemie: návody do cvičení z kvalitativní anorganické a organické analýzy. Praha: Karolinum, 2010, 89 s. ISBN 978-80-246-0121-2.

  • Karlíček, Rolf. Analytická chemie: návody do cvičení z kvantitativní analýzy. Praha: Karolinum, 2010, 97 s. ISBN 978-80-246-0221-9.

  • Karlíček, Rolf. Protokoly ke kvalitativní anorganické a organické analýze a ke kvantitativní analýze. Praha: Karolinum, 2009, 107 s. ISBN 978-80-246-1720-6.

  • Analytická chemie - Moodle [online]. Dostupné z: http://dl1.cuni.cz/enrol/index.php?id=1332

  • Analytická chemie - studijní materiály [online]. Dostupné z: https://intranet.faf.cuni.cz/Studijni-materialy/KACH/

Doporučená:

  • Skoog, Douglas A.. Skoog and West's fundamentals of analytical chemistry. [Belmont, Calif.]: Brooks/Cole Cengage Learning, 2013, 958 s. ISBN 978-1-285-05624-1.

  • Christian, Gary D., Dasgupta, Purnendu K. Schug, Kevin. Analytical chemistry. Hoboken: Wiley, 2014, 826 s. ISBN 978-0-470-88757-8.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (22.08.2025)
Syllabus -

Syllabus – Analytical Chemistry

Types of Analytical reactions

  • Basic types of analytical reactions. Selectivity and sensitivity of analytical reactions.

  • Analytical protolytic reactions. Buffers in analytical chemistry. Acids and bases in non-aqueous solvents.

  • Analytical complex-formation reactions and their equilibrium. Use of complex-formation reactions in analytical chemistry. Masking. Organic complex-formation reagents.

  • Analytical oxidation-reduction (redox) reactions, their equilibrium and kinetics. Catalytic and induced reaction.

  • Analytical precipitation reactions and their equilibrium. Extraction equilibrium.

Qualitative inorganic analysis

  • Detection and determination of selected cations and anions. Colour, precipitation and oxido-reduction identification reaction, group, selective and specific reagents.

  • Evaluation of qualitative analysis of simple inorganic compound.

Analysis of organis compounds

  • Methods of organic analysis. Elemental analysis: detection of C, H, N, S, O, and halogens in organic compounds. Determination of C, H, N, S, O, and halogens.

  • Functional analysis: detection of alcohols, phenols, amines, aldehydes and ketones, carboxylic acids, esters, amides, sulphonamides, sulphonic acids, hydrocarbons, nitro- and nitroso-compounds.

  • Identification and determination of hydrocarbons, alcohols, phenols, amines, aldehydes and ketones, carboxylic acids, esters, amides, sulphonamides, sulphonic acids, nitro- and nitroso-compounds. Analysis of organic salts.

  • Solubility of organic compounds - classification system, structure-solubility relationships and their analytical use.

Direct Methods of quantitative analysis

  • General procedures in quantitative analysis. Sample pre-treatment. Processing and evaluation of results of quantitative analysis.

  • Fundamentals and types of gravimetric methods.

  • Titrimetric (volumetric) analysis; direct titration, indirect titration, back-titration. Concentration of volumetric solutions and their standardisation.

  • Acidimetry and alkalimetry, acid-base indicators, titration in non-aqueous solvents. Chelatometry, chelatometric indicators. Permanganatometry. Iodimetry. Bromatometry. Dichromatometry. Argentometry. Mercurimetry.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (22.08.2025)
Learning resources - Czech

E-learning Moodle: Analytická chemie https://dl1.cuni.cz/enrol/index.php?id=1332

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (22.08.2025)
Learning outcomes -

The subject Analytical Chemistry builds on the knowledge and skills acquired in the subjects: General and Inorganic Chemistry and Organic Chemistry I.

After completing the subject, students will be able to use the following terms in the correct context towards the analytical evaluation of inorganic and organic substances: group, selective and specific proof reactions for inorganic cations and anions; use of the solubility of group reaction products for separation; proof of organic compounds including elemental analysis and proof of functional groups; use of knowledge of solubility and acid-base properties of organic substances for proof of functional groups; volumetric analysis using various chemical principles, method of determining the equivalence point, standardization of volumetric solutions and calculation of content determination using direct, indirect and back titration.

Learning outcomes:

Based on the acquired knowledge and skills, students will:

  • describe the general procedure for proof of cations and list group reagents for proof of cations including the principle of proof reactions;
  • describe the general procedure for anion proofs and list the group reagents for anion proofs, describe the principles and evaluation of proof reactions;
  • describe the general procedure for the analysis of organic substances, including the difference between inorganic and organic qualitative analysis, describe the procedure for elemental analysis, solubility test and proof of functional groups of organic substances;
  • describe the general procedure for titration with visual indication of the equivalence point, list the three basic types of titrations with regard to the speed and conditions of the chemical reaction, explain the procedure for standardizing volumetric solutions and propose the principle of titration for determining the content of a simple inorganic substance, calculate the content of the substance being determined
Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (22.08.2025)