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Course, academic year 2024/2025
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Molecular Biology - GF144
Title: Molekulární biologie
Guaranteed by: Department of Biochemical Sciences (16-16160)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2016
Semester: winter
Points: 0
E-Credits: 3
Examination process: winter s.:written
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: not taught
Language: Czech
Teaching methods: full-time
Level:  
Explanation: (F 3.r.)
Note: deregister from the exam date if a requisite was not fulfilled
course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: prof. PharmDr. Tomáš Šimůnek, Ph.D.
Classification: Pharmacy >
Co-requisite : GF283, GF284
Incompatibility : GF296
Interchangeability : GF296
Is incompatible with: GF296
Is interchangeable with: GF296
Examination dates   Schedule   
Annotation -
Molecular biology deals with the study of the cellular processes on molecular level. The main focus of this subject is description of heredity-related biological macromolecules (DNA, RNA, proteins), their mutual interactions and regulation of their function. Furthermore, the subject scope is extended towards explanation of the most important experimental molecular techniques, basics of clinical genetics and molecular diagnostics, as well as application of genetic engineering (recombinant DNA technology) in Medicine and Pharmacy.
Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
Literature -

Alberts B. et al. Molecular biology of the cell. 4th edition, Garland Science, 2002.

Brown TA. et al. Genomes. 2nd edition, BIOS Scientific Publishers Ltd, 2002.

Miesfeld RL. Applied Molecular Genetics, Wiley-Liss, Inc., 1999.

Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
Teaching methods -

Interactive lectures

Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
Requirements to the exam -

Molecular Biology - Examination Topics

Pharmacy - Winter term 2007/2008

1. Model organisms in molecular biology

2. Structure and organisation of prokaryotic and eukaryotic genomes

3. DNA replication

4. Cell cycle, cell death, cell cycle disturbancies

5. Genetic mutations

6. Genetic repair, recombination

7. Structure and types of RNA

8. Transcription + posttranscriptional modifications of RNA

9. Genetic code, ribosomes, translation

10. Posttranslational modifications, protein degradation

11. Regulation of gene expression in prokaryotes

12. Regulation of gene expression in and eukaryotes

13. Isolation of nucleic acids

14. Overview of enzymes used in experimental molecular biology

15. PCR

16. Gel electrophoresis

17. DNA sequencing

18. Hybridisation, blotting

19. Molecular cloning, methods of gene transfer

20. Hereditary diseases

Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
Syllabus -

Model organisms in molecular biology

Structure and organisation of prokaryotic and eukaryotic genomes

DNA replication

Cell cycle, cell death, cell cycle disturbancies

Genetic mutations

Genetic repair, recombination

Structure and types of RNA

Transcription + posttranscriptional modifications of RNA

Genetic code, ribosomes, translation

Posttranslational modifications, protein degradation

Regulation of gene expression in prokaryotes

Regulation of gene expression in and eukaryotes

Isolation of nucleic acids

Overview of enzymes used in experimental molecular biology

Polymerase chain reaction and its modifivations

Gel electrophoresis

DNA sequencing

Hybridisation, blotting

Molecular cloning, methods of gene transfer

Hereditary diseases

Molecular diagnostics

Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
Entry requirements -

Students may attend Molecular Biology only after succesful passing of the exam from General Biology.

Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
 
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