Electrophoretic methods in the analysis of biologically active compounds - GDBM03
Title: Elektroforetické metody v analýze biologicky aktivních látek
Guaranteed by: Department of Analytical Chemistry (16-16140)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2024
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 0/0, Ex [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Note: course is intended for doctoral students only
enabled for web enrollment
you can enroll for the course in winter and in summer semester
Guarantor: prof. RNDr. Dalibor Šatínský, Ph.D.
Interchangeability : GDZCH03
Is interchangeable with: GDZCH03
Examination dates   WS schedule   
Annotation -
Development trends in electrophoretic methods, miniaturization, chip electrophoresis. Electrophoresis in proteomic studies. Non-aqueous capillary electrophoresis and its applications. Selected applications of capillary electrophoresis - separation of peptides and proteins, chiral separation. On-line "stacking" techniques in capillary electrophoresis for the analysis of biological materials. Micellar electrokinetic chromatography. Connection of capillary electrophoresis with advanced detection techniques and mass spectrometry.
Last update: Šatínský Dalibor, prof. RNDr., Ph.D. (28.02.2018)
Literature - Czech

Povinná:

  • Garcia, Carlos D. (ed.). Capillary electrophoresis and microchip capillary electrophoresis principles, applications, and limitations. Hoboken, New Jersey: Wiley, 2013, 394 s. ISBN 978-0-470-57217-7.

  • Nicola Volpi . Capillary Electrophoresis of Biomolecules . London: Humana Press , 2013, s. ISBN 978-1-62703-295-7.

Doporučená:

  • M.G. Khaledi . High Performance Capillary Electrophoresis – Theory, Techniques and Applications, . Amsterdam : Willey, 1998, s. ISBN .

  • . . In Electrophoresis, Journal of Separation Sciences, Journal of Chromatography A. (): -.

  • Web of Knowledge

  • sciencediretc

Last update: prepocet_literatura.php (14.08.2025)
Requirements to the exam -

 

Theoretical aspects of electromigration techniques.

Detection techniques in capillary electrophoresis (CE).

CE in drug and macromolecular analyzes, proteomic analyzes.

Modern trends in electromigration techniques.

The CE for on-line pre-concentration of trace substances.

Trends in micellar electrophoresis and capillary electrochromatography.

Trends in CE coupling with Mass Detection for Biomolecular Analysis.

Last update: Šatínský Dalibor, prof. RNDr., Ph.D. (28.02.2018)