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Phytochemical Methods Laboratory I - GAPL003

Title:	Laboratoř z fytochemických metod I.
Guaranteed by:	Department of Pharmacognosy and Pharmaceutical Botany (16-16230)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2024
Semester:	winter
Points:	0
E-Credits:	3
Examination process:	winter s.:
Hours per week, examination:	winter s.:0/42, C [HS]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Level:

Guarantor:	doc. PharmDr. Jakub Chlebek, Ph.D.

Examination dates WS schedule

Annotation -

The aim of the course is to introduce students to isolation and analytical procedures required for working with plant material to successful isolate of various constituents and determination of the content of selected secondary metabolites using modern analytical methods. Students will learn the process of selecting and processing the starting material from extraction, separation to identification of its constituent metabolites and the possibility of modifying the structures of natural substances to increase stability, detection and biological activity, or bioavailability. The practical exercises also include in silico evaluation of isolated compounds.

Last update: Chlebek Jakub, doc. PharmDr., Ph.D. (08.05.2025)

Course completion requirements -

100% attendance for practical classes.

Last update: Chlebek Jakub, doc. PharmDr., Ph.D. (08.05.2025)

Literature - Czech

Povinná:

Successful Flash Chromatography A White Paper from Biotage [online]. Dostupné z: https://www.biotage.com/hubfs/Archive/UngatedPDF/pps490.v3_-_white_paper_successful_flash_chromatography.pdf

Doporučená:

Opletal, L., Drašar, P.. Fytochemické metody. 1. Izolace obsahových látek (laboratorní technika). Praha : Karolinum, 1994, s. ISBN .

Ikan, R.. Natural Products. A Laboratory Guide.. London: Academic Press, 1991, s. ISBN .

Cannell, R. J. P.. Natural Products Isolation.. Totowa : Humana Press, 1998, s. ISBN .

Harborne J.B.. Phytochemical Methods: a guide to modern techniques of plant analysis.. London: Chapman and Hall, 1998, s. ISBN .

Colegate, M.S., Molyneux, R.J.. Bioactive natural products: detection, isolation, and structural determination.. Boca Raton: CRC Pres, Taylor & Francis Group, 2008, s. ISBN 978042912.

Last update: Chlebek Jakub, doc. PharmDr., Ph.D. (15.01.2025)

Teaching methods - Czech

Praktická cvičení.

Last update: Chlebek Jakub, doc. PharmDr., Ph.D. (08.05.2025)

Syllabus -

1. Collection and treatment of plant material

2. Preparation of summary plant extracts

3. Preparation of specific extracts according to the type of secondary plant metabolites I.

4. Preparation of specific extracts according to the type of secondary plant metabolites II.

5. TLC analysis of extracts (chemical identification of the components)

6. Flash chromatography

7. Evaluation of fractions obtained from Flash chromatography

Last update: Chlebek Jakub, doc. PharmDr., Ph.D. (08.05.2025)

Learning outcomes -

Theoretical knowledge of the subject Phytochemical Methods (GAPL002). Furthermore, knowledge of the basics of organic and analytical chemistry, including instrumental methods.
The subject Laboratory of Phytochemical Methods I builds on the knowledge and skills acquired in the subjects Phytochemical Methods, Chemistry of Natural Substances I. A graduate of the practical exercises in phytochemical methods knows the strategy of selecting and processing plant material from its collection, preparation of extracts with their subsequent analysis (TLC, chemical evidence reactions) and separation of compounds from prepared extracts by chromatographic methods. He is able to fractionate extracts by Flash chromatography (method development, setting parameters in the software of the instrument for separating compounds, as well as subsequent processing of fractions, including evaluation of the separation and composition of the obtained fractions).

Learning outcomes:
Based on the acquired knowledge and skills, students will:

select and assess the strategy for selecting plant material and its processing for the isolation of substances
define factors influencing the extraction processes of compounds from biogenic material
know the principles of separation and detection of substances using TLC and HPTLC, chemical evidence reactions
design a method for the separation of substances using Flash chromatography, including method development, setting parameters in the software, including evaluation of the separation and composition of the obtained fractions

Last update: Chlebek Jakub, doc. PharmDr., Ph.D. (08.05.2025)

Entry requirements -

Theoretical knowledge of the subject Phytochemical Methods (GAPL002). Furthermore, knowledge of the basics of organic and analytical chemistry, including instrumental methods.

Last update: Chlebek Jakub, doc. PharmDr., Ph.D. (08.05.2025)