Subjects

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Molecular Biology - GAF296

Title:	Molekulární biologie
Guaranteed by:	Department of Biochemical Sciences (16-16160)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2021
Semester:	summer
Points:	0
E-Credits:	4
Examination process:	summer s.:written
Hours per week, examination:	summer s.:28/0, Ex [HS]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	cancelled
Language:	English
Teaching methods:	full-time
Level:
Note:	deregister from the exam date if a requisite was not fulfilled enabled for web enrollment

Guarantor:	prof. PharmDr. Tomáš Šimůnek, Ph.D.
Co-requisite :	GAF141
Incompatibility :	GAF144
Interchangeability :	GAF144, GAF370
Is incompatible with:	GAF144
Is interchangeable with:	GAF144

Examination dates Schedule

Annotation -

Molecular biology deals with the study of the cellular processes on molecular level. The main focus of this subject is description of heredity-related biological macromolecules (DNA, RNA, proteins), their mutual interactions and regulation of their function. Furthermore, the subject scope is extended towards explanation of the most important experimental molecular techniques, basics of clinical genetics and molecular diagnostics, as well as application of genetic engineering (recombinant DNA technology) in Medicine and Pharmacy.

Last update: Šimůnek Tomáš, prof. PharmDr., Ph.D. (22.12.2017)

Course completion requirements -

Exam Information

Written test, consists of two parts

Part A: 30 statements - to distinguish between correct and incorrect (maximum 30 points)

Part B: 5 topics/questions to answer by writing (maximum 15 points)

Last update: Šimůnek Tomáš, prof. PharmDr., Ph.D. (22.12.2017)

Literature -

Obligatory:

Alberts B.. Základy molekulární biologie . : Espero Publishing, 2005, s. ISBN .

Šmarda J.. Metody molekulární biologie . . : Masarykova Univerzita, 2005, s. ISBN .

Zima T.. Laboratorní diagnostika.. : Galén, 2013, s. ISBN .

Last update: prepocet_literatura.php (19.09.2024)

Syllabus -

Lectures

Model organisms in molecular biology

Structure and organisation of prokaryotic and eukaryotic genomes

DNA replication

Cell cycle, cell death, cell cycle disturbancies

Genetic mutations

Genetic repair, recombination

Structure and types of RNA

Transcription + posttranscriptional modifications of RNA

Genetic code, ribosomes, translation

Posttranslational modifications, protein degradation

Regulation of gene expression in prokaryotes

Regulation of gene expression in and eukaryotes

Isolation of nucleic acids

Overview of enzymes used in experimental molecular biology

Polymerase chain reaction and its modifivations, real-time PCR

Gel electrophoresis

DNA sequencing

Hybridisation, blotting

Molecular cloning, methods of gene transfer

Hereditary diseases

Molecular diagnostics

Introduction to proteomics

Bioinformatics

Last update: Šimůnek Tomáš, prof. PharmDr., Ph.D. (22.12.2017)