Cell Biology - GAF283
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Examination dates Schedule
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The main goal of Cell Biology is to reveal to the students how the living cell works and teach them how to handle the microscope. Both will give them the background for the subjects in the upcoming years. For that, we introduce them to the structure and function of every single compartment of the cell and its chemical composition. Other areas such as cell communication, cell division, and regulation of the cell cycle are studied.
Themes: Structure of prokaryotic and eukaryotic cells, membranes, membrane organelles, DNA and chromosomes, cell communication, cell cycle, reproduction of organisms.
Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
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The course consists of lectures and practical classes. The practical classes are completed by getting a credit, which is a necessary condition for access to the final exam test.
The credit will be recognized after fulfillment of the following conditions: 1) 100% presence in all practical classes. One PC can be exused in case of serious healthy of family issues that must be confirmed by a doctor or a lawyer (with regards to the COVID-19 situation). 2) Deliver the Laboratory manual with filled protocols to be checked on time 3) Practical part - to perform one of the tasks from the previous practical classes (fill in the protocol) and answer 3 theoretical questions from practical classes.
The final exam test is in the written form and is composed of 35 questions (theory from lectures). Types of questions: single best answer, matching, image description. limit for passing the test: 70% of correct answers evaluation: excellent: 100 % - 90 % very good: 89 % - 80 % good: 79 % - 70 % Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
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Obligatory:
Last update: prepocet_literatura.php (19.09.2024)
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Lectures - theory Moodle - videos, "quizzes", "questions and answers", make-up own "glossary" Practical classes - microscopy, observation of plant and animal cells, function of the organelles, osmotic effects in plant and animal cells, cell cycle, cell counting Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
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The exam test is composed of 35 questions (only from theory).
Types of questions: single best answer, matching, image description.
Limit for passing the test: 70% of correct answers evaluation: excellent: 100 % - 90 % very good: 89 % - 80 % good: 79 % - 70 %
The student should show knowledge across the subject of Cell Biology, specifically in the following areas: 1. Definition and general characteristics of cell 2. Structure of prokaryotic cell3. Structure of eukaryotic cell 4. Cell nucleus 5. Structure of DNA 6. Chromosome structure in prokaryotes and viruses 7. Chromosome structure in eukaryotes 8. Replication, transcription and translation 9. Basic principles of light microscopy, fixation and staining, fluorescent microscopy 10. Structure of living systems – water, inorganic substances, low molecular weight organic molecules (saccharides, organic acids, amino acids, nucleotides, phospholipids) 11. Structure of living systems – high molecular weight organic molecules (polysaccharides, proteins, nucleic acids) 12. Membrane structure 13. Cytoplasmic membrane 14. Membrane transport (carrier proteins, ion channels) 15. Mitochondria (structure, cellular respiration) 16. Chloroplasts (structure, photosynthesis) 17. Endoplasmic reticulum 18. Golgi apparatus 19. Endosomes 20. Lysosomes 21. Peroxisomes 22. Intracellular vesicular transport 23. General principles of cell communications 24. Receptors (basic characteristics, signaling cascade) 25. Ion-channel–coupled receptors 26. G-protein–coupled receptors 27. Enzyme–coupled receptors 28. Microtubules 29. Actin filaments 30. Intermediate filaments 31. Cell cycle 32. Mitosis 33. Meiosis 34. Cell cycle control system 35. Cell differentiation 36. Cell death 37. Cell stress 38. Asexual reproduction 39. Spermatogenesis 40. Oogenesis 41. Fertilization Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
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Cell structure · definition and general characteristics of cell (cell theory) · structure of prokaryotic cell · structure of eukaryotic cell (plant and animal)
Chemical composition of living systems · water and inorganic substances · low molecular weight organic molecules: saccharides, organic acids, amino acids, nucleotides, phospholipids · high molecular weight organic molecules: polysaccharides, proteins, nucleic acids
Nucleic acids and chromosomes I · structure of DNA · chromosome structure in prokaryotes and viruses · chromosome structure in eukaryotes
Nucleic acids and chromosomes II · replication · transcription · translation
Membranes I · membrane structure o lipid bilayer o molecular structure of membranes – lipids, proteins, saccharides · cytoplasmic membrane
Membranes II · membrane transport · carrier proteins · ion channels and the electrical properties of membranes
Membrane organelles I · mitochondria o the structure o cellular respiration – Krebs cycle, electron-transport chain, oxidative phosphorylation · chloroplasts o the structure o photosynthesis – light reactions and dark reactions
Membrane organelles II · endoplasmic reticulum · Golgi apparatus · endosomes · lysosomes · peroxisomes · intracellular vesicular transport o transport from the endoplasmic reticulum through the Golgi apparatus o transport from the trans Golgi network to lysosomes o transport into the cell from the plasma membrane: endocytosis o transport from the trans Golgi network to the cell exterior: exocytosis
Cell communications · general principles of cell communication · signal molecules · receptors o ion-channel–coupled receptors o G-protein–coupled receptors o enzyme–coupled receptors
Cell division · cell cycle - four phases (G1, S, G2, M) · mitosis - prophase, prometaphase, metaphase, anaphase, telophase, cytokinesis · meiosis
Cell cycle control system · regulation of the cell cycle · cell differentiation · cell death
Reproduction of organisms
Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
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