SubjectsSubjects(version: 964)
Course, academic year 2024/2025
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Cell Biology - GAF283
Title: Cell Biology
Guaranteed by: Department of Biological and Medical Sciences (16-16150)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2022
Semester: winter
Points: 0
E-Credits: 5
Examination process: winter s.:written
Hours per week, examination: winter s.:28/14, C+Ex [HS]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: not taught
Language: English
Teaching methods: full-time
Level:  
Explanation: 1.roč. F
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Mgr. Zuzana Svobodová, Ph.D.
Interchangeability : GAF007, GAF387
Is co-requisite for: GAF144, GAF366, GAF129, GAF284
Is incompatible with: GAF387
Is interchangeable with: GAF387, GAF007
Examination dates   Schedule   
Annotation -
The main goal of Cell Biology is to reveal to the students how the living cell works and teach them how to handle the microscope. Both will give them the background for the subjects in the upcoming years. For that, we introduce them to the structure and function of every single compartment of the cell and its chemical composition. Other areas such as cell communication, cell division, and regulation of the cell cycle are studied. Themes: Structure of prokaryotic and eukaryotic cells, membranes, membrane organelles, DNA and chromosomes, cell communication, cell cycle, reproduction of organisms.
Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
Course completion requirements -

The course consists of lectures and practical classes. The practical classes are completed by getting a credit, which is a necessary condition for access to the final exam test.

 

The credit will be recognized after fulfillment of the following conditions:

1) 100% presence in all practical classes. One PC can be exused in case of serious healthy of family issues that must be confirmed by a doctor or a lawyer (with regards to the COVID-19 situation).

2) Deliver the Laboratory manual with filled protocols to be checked on time

3) Practical part - to perform one of the tasks from the previous practical classes (fill in the protocol) and answer 3 theoretical questions from practical classes.

 

The final exam test is in the written form and is composed of 35 questions (theory from lectures).

Types of questions: single best answer, matching, image description.

limit for passing the test: 70% of correct answers

evaluation: excellent: 100 % - 90 %

 very good: 89 % - 80 %

 good: 79 % - 70 %

Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
Literature -

Obligatory:

Last update: prepocet_literatura.php (19.09.2024)
Teaching methods -

Lectures - theory

Moodle - videos, "quizzes", "questions and answers", make-up own "glossary"

Practical classes - microscopy, observation of plant and animal cells, function of the organelles, osmotic effects in plant and animal cells, cell cycle, cell counting

Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
Requirements to the exam -

The exam test is composed of 35 questions (only from theory).

 

Types of questions: single best answer, matching, image description.

 

Limit for passing the test: 70% of correct answers evaluation:

excellent: 100 % - 90 %

very good: 89 % - 80 %

good: 79 % - 70 %

 

The student should show knowledge across the subject of Cell Biology, specifically in the following areas:

1. Definition and general characteristics of cell

2. Structure of prokaryotic cell3. Structure of eukaryotic cell

4. Cell nucleus

5. Structure of DNA

6. Chromosome structure in prokaryotes and viruses

7. Chromosome structure in eukaryotes

8. Replication, transcription and translation

9. Basic principles of light microscopy, fixation and staining, fluorescent microscopy

10. Structure of living systems – water, inorganic substances, low molecular weight organic molecules (saccharides, organic acids, amino acids, nucleotides, phospholipids)

11. Structure of living systems – high molecular weight organic molecules (polysaccharides, proteins, nucleic acids)

12. Membrane structure

13. Cytoplasmic membrane

14. Membrane transport (carrier proteins, ion channels)

15. Mitochondria (structure, cellular respiration)

16. Chloroplasts (structure, photosynthesis)

17. Endoplasmic reticulum

18. Golgi apparatus

19. Endosomes

20. Lysosomes

21. Peroxisomes

22. Intracellular vesicular transport

23. General principles of cell communications

24. Receptors (basic characteristics, signaling cascade)

25. Ion-channel–coupled receptors

26. G-protein–coupled receptors

27. Enzyme–coupled receptors

28. Microtubules

29. Actin filaments

30. Intermediate filaments

31. Cell cycle

32. Mitosis

33. Meiosis

34. Cell cycle control system

35. Cell differentiation

36. Cell death

37. Cell stress

38. Asexual reproduction

39. Spermatogenesis

40. Oogenesis

41. Fertilization

Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
Syllabus -

Cell structure

·         definition and general characteristics of cell (cell theory)

·         structure of prokaryotic cell

·         structure of eukaryotic cell (plant and animal)

 

Chemical composition of living systems

·         water and inorganic substances

·         low molecular weight organic molecules: saccharides, organic acids, amino acids, nucleotides, phospholipids

·         high molecular weight organic molecules: polysaccharides, proteins, nucleic acids

 

Nucleic acids and chromosomes I

·         structure of DNA

·         chromosome structure in prokaryotes and viruses

·         chromosome structure in eukaryotes

 

Nucleic acids and chromosomes II

·         replication

·         transcription

·         translation

 

Membranes I

·      membrane structure

o   lipid bilayer

o   molecular structure of membranes – lipids, proteins, saccharides

·      cytoplasmic membrane

 

Membranes II

·           membrane transport

·           carrier proteins

·           ion channels and the electrical properties of membranes

 

Membrane organelles I

·         mitochondria

o   the structure

o   cellular respiration – Krebs cycle, electron-transport chain, oxidative phosphorylation

·         chloroplasts

o   the structure

o   photosynthesis – light reactions and dark reactions

 

Membrane organelles II

·         endoplasmic reticulum

·         Golgi apparatus

·         endosomes

·         lysosomes

·         peroxisomes

·         intracellular vesicular transport

o   transport from the endoplasmic reticulum through the Golgi apparatus

o   transport from the trans Golgi network to lysosomes

o   transport into the cell from the plasma membrane: endocytosis

o   transport from the trans Golgi network to the cell exterior: exocytosis

 

Cell communications

·         general principles of cell communication

·         signal molecules

·         receptors

o   ion-channel–coupled receptors

o   G-protein–coupled receptors

o   enzyme–coupled receptors

 

Cell division

·         cell cycle - four phases (G1, S, G2, M)

·         mitosis - prophase, prometaphase, metaphase, anaphase, telophase, cytokinesis

·         meiosis

 

Cell cycle control system 

·         regulation of the cell cycle

·         cell differentiation

·         cell death

 

Reproduction of organisms

  • asexual reproduction
    • characteristics
    • binary division, vegetative reproduction, amitosis
  • sexual reproduction
    • characteristics
    • gametogenesis - spermatogenesis, oogenesis, fertilization
Last update: Svobodová Zuzana, Mgr., Ph.D. (24.09.2021)
 
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