Biotechnology - GAF231

• Title: Biotechnology
• Guaranteed by: Department of Pharmacognosy and Pharmaceutical Botany (16-16230)
• Faculty: Faculty of Pharmacy in Hradec Králové
• Actual: from 2024
• Semester: summer
• Points: 0
• E-Credits: 3
• Examination process: summer s.:written
• Hours per week, examination: summer s.:28/14, C+Ex [HS]
• Capacity: unlimited / unlimited (unknown)
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Explanation: (F 3.r.)
• Note: deregister from the exam date if a requisite was not fulfilled; course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: doc. PharmDr. Tomáš Siatka, CSc.
• Co-requisite : GAF370
• In complex pre-requisite: GAF1004

Annotation

English

A basic course in biotechnology. The subject describes and explains concepts and theoretical principles underlying the biotechnology-based development and manufacturing of biologically active natural products as well as the other biotechnological applications relevant to the human health. Special attention is paid to biopharmaceuticals (manufacturing and applications).

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Czech

Seznámení se základy biotechnologie se zaměřením na teoretické principy a praktické použití biotechnologických metod využívaných při získávání a výrobě farmaceuticky významných látek, a na uplatnění biotechnologických postupů v oblastech souvisejících s lidským zdravím. Zvláštní pozornost je věnována bioléčivům (výroba a aplikace).

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Course completion requirements

English

A requirement for passing credit is fulfiment of the following conditions:

• presence at all seminars, fulfilment of seminar papers, and attendace at at least seven lectures before seminars

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Czech

Podmínkou udělení zápočtu je splnění následujících podmínek:

• účast na všech seminářích, splnění seminárních prací, účast na nejméně sedmi přednáškách do začátku semináře

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Literature

English

Obligatory:

• null. . In Kristiansen, B. Ratledge, Colin (eds.). Basic biotechnology . Cambridge, U.K. ; New York: Cambridge University Press, 2006, s. -. ISBN 0-521-84031-7..

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Czech

Povinná:

• null. . In Kristiansen, B. Ratledge, Colin (eds.). Basic biotechnology . Cambridge, U.K. ; New York: Cambridge University Press, 2006, s. -. ISBN 0-521-84031-7..

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Teaching methods

English

Lectures and seminars.

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Czech

Přednášky a semináře.

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Requirements to the exam

English

To pass credit.

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Czech

Získání zápočtu.

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Syllabus

English

Biotechnology.

• Basic terms.

• History of biotechnology.

Industrial biotechnology.

• Biotechnological process.

• Industrial microorganisms.

• Fermentation equipment, mixing, aeration, sterilization.

• Culture media.

• Microbial growth and product formation.

• Fermentation monitoring and control.

• Downstream processing.

Recombinant technology.

• Protoplast and cell fusion.

• Recombinant DNA technology.

• Protein engineering.

• Production of recombinant proteins.

Enzyme technology.

• Enzymes and cells as biocatalysts.

• Immobilized biocatalysts.

• Industrial biotransformations.

Plant biotechnology.

• Plant tissue culture.

• Transgenic plants.

Animal biotechnology.

• Animal cell culture.

• Hybridoma technology.

• Transgenic animals.

Pharmaceuticals produced by means of biotechnological methods.

• Small molecules and biopharmaceuticals.

• Antibiotics.

• Immunosuppressant.

• Alkaloids.

• Enzymes.

• Enzyme inhibitors.

• Cytokines.

• Monoclonal antibodies.

• Fusion proteins.

• Vitamins.

• Amino acids, dipeptides.

• Organic acids.

• Oligosaccharides and polysaccharides.

• Living cells and organisms.

• Vaccines.

• Genes.

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Czech

Biotechnologie.

• Základní pojmy.

• Historický vývoj biotechnologie.

Průmyslová mikrobiologie.

• Biotechnologický proces.

• Průmyslové mikroorganismy.

• Kultivační zařízení, míchání, vzdušnění, sterilizace.

• Kultivační média.

• Růst a tvorba produktů.

• Měření, kontrola a regulace biotechnologických procesů.

• Izolace a čištění produktů.

Rekombinantní technologie

• Fúze buněk a protoplastů.

• Technologie rekombinantní DNA.

• Proteinové inženýrství.

• Produkce rekombinantních proteinů.

Enzymové technologie

• Enzymy a buňky jako biokatalyzátory

• Imobilizované biokatalyzátory.

• Biotransformace v průmyslu.

Rostlinné biotechnologie

• Explantátové kultury.

• Geneticky modifikované rostliny.

Živočišné biotechnologie

• Buněčné kultivační technologie.

• Hybridomové technologie.

• Geneticky modifikovaná zvířata.

Léčiva získávaná biotechnologickými metodami.

• Malé molekuly a biologická léčiva.

• Antibiotika.

• Imunosupresiva.

• Alkaloidy.

• Enzymy.

• Enzymové inhibitory.

• Peptidové a proteinové hormony.

• Cytokiny.

• Monoklonální protilátky.

• Fúzní proteiny.

• Vitaminy.

• Aminokyseliny, dipeptidy.

• Organické kyseliny.

• Oligosacharidy, polysacharidy.

• Živé buňky a organismy.

• Vakcíny.

• Geny.

Last update: Siatka Tomáš, doc. PharmDr., CSc. (19.02.2025)

Learning outcomes

The course in Biotechnology builds on the knowledge and skills gained in the following subjects: Cell Biology, Microbiology, General Biochemistry, Molecular Biology and Genetics, Pathological Physiology for Pharmacists.

Students get acquainted with the basics of biotechnology with a focus on concepts and theoretical principles underlying the biotechnology-based development and manufacturing of biologically active natural products, as well as the other biotechnological applications relevant to human health. After completing the course, students will be able to use the following terms in the correct context: biotechnological process, upstream, downstream, biosynthesis, biotransformation, industrial microorganism, recombinant DNA technology, vector, transformation, transfection, immobilized biocatalyst, protein engineering, plant tissue culture, genetically modified plant, cell culture technology, hybridoma technology, monoclonal antibody engineering, genetically modified animal, small molecule, biological drug, antibiotic, immunosuppressant, hormone, monoclonal antibody, fusion protein, polysaccharide, subunit vaccine.

Learning outcomes:

Based on the knowledge gained, students:

- define the importance of pharmaceutical biotechnology

- are familiar with the scientific literature dealing with biotechnology and search for relevant information from the specialized literature

- define the term biotechnological process, list its components, explain the principles of the methods and procedures it uses, and evaluate the factors that influence its course

- characterize basic types of vectors and the possibilities of their use

- explain the principles of methods for introducing recombinant DNA into various cell types

- compare the advantages and disadvantages of different methods of immobilizing biocatalysts

- explain the differences in the cultivation of plant and animal cells in vitro

- explain the basic steps of hybridoma technology

- describe methods for obtaining genetically modified animals and plants

- list the basic groups of drugs obtained by biotechnological methods, understand the mechanism of their action and therapeutic use

Last update: Siatka Tomáš, doc. PharmDr., CSc. (28.03.2025)