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Molecular biology deals with the study of the cellular processes on molecular level. The main focus of this subject is description of heredity-related biological macromolecules (DNA, RNA, proteins), their mutual interactions and regulation of their function. Furthermore, the subject scope is extended towards explanation of the most important experimental molecular techniques, basics of clinical genetics and molecular diagnostics, as well as application of genetic engineering (recombinant DNA technology) in Medicine and Pharmacy.
Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
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Alberts B. et al. Molecular biology of the cell. 4th edition, Garland Science, 2002. Brown TA. et al. Genomes. 2nd edition, BIOS Scientific Publishers Ltd, 2002. Miesfeld RL. Applied Molecular Genetics, Wiley-Liss, Inc., 1999. Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
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Interactive lectures Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
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Molecular Biology - Examination Topics
Pharmacy - Winter term 2007/2008
1. Model organisms in molecular biology 2. Structure and organisation of prokaryotic and eukaryotic genomes 3. DNA replication 4. Cell cycle, cell death, cell cycle disturbancies 5. Genetic mutations 6. Genetic repair, recombination 7. Structure and types of RNA 8. Transcription + posttranscriptional modifications of RNA 9. Genetic code, ribosomes, translation 10. Posttranslational modifications, protein degradation 11. Regulation of gene expression in prokaryotes 12. Regulation of gene expression in and eukaryotes 13. Isolation of nucleic acids 14. Overview of enzymes used in experimental molecular biology 15. PCR 16. Gel electrophoresis 17. DNA sequencing 18. Hybridisation, blotting 19. Molecular cloning, methods of gene transfer 20. Hereditary diseases
Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
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Model organisms in molecular biology Structure and organisation of prokaryotic and eukaryotic genomes DNA replication Cell cycle, cell death, cell cycle disturbancies Genetic mutations Genetic repair, recombination Structure and types of RNA Transcription + posttranscriptional modifications of RNA Genetic code, ribosomes, translation Posttranslational modifications, protein degradation Regulation of gene expression in prokaryotes Regulation of gene expression in and eukaryotes Isolation of nucleic acids Overview of enzymes used in experimental molecular biology Polymerase chain reaction and its modifivations Gel electrophoresis DNA sequencing Hybridisation, blotting Molecular cloning, methods of gene transfer Hereditary diseases Molecular diagnostics Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
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Students may attend Molecular Biology only after succesful passing of the exam from General Biology. Last update: SIMUNEKT/FAF.CUNI.CZ (10.06.2008)
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