Advanced Techniques in Microbiology - FV139

• Title: Advanced Techniques in Microbiology
• Guaranteed by: Department of Clinical Microbiology (15-560)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2023
• Semester: summer
• Points: 0
• E-Credits: 1
• Examination process: summer s.:
• Hours per week, examination: summer s.:9/6, C [HS]
• Capacity: 9
• Min. number of students: 5
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: MUDr. Miroslav Fajfr, Ph.D.
• Comes under: General medicine - ELECTIVE subjects
• Pre-requisite : {Malá skupina pro FV039}
• Incompatibility : FA0105111, FG50026
• Interchangeability : FG50026

Annotation

Last update: Eva Dyntarová (07.02.2024)

Advanced molecular techniques are now routinely used in the microbiological laboratory. Student of this course will thus learn common molecular methods, such as PCR and its modifications, MALDI-TOF, next generation sequencing (NGS) and others utilized in microbiology. In addition, the students will acquire knowledge about specific incorporation of these methods in the clinical diagnostic procedures. During the first practical, the students will perform an isolation of nucleic acids, PCR setup and subsequent detection using gel electrophoresis. The second practical will aim at performing an identification of bacterial cultures using MALDI-TOF.

Requirements to the exam

Last update: MUDr. Miroslav Fajfr, Ph.D. (07.02.2024)

Credit:

1.  full attendance required (absences are not allowed)

Syllabus

Last update: Eva Dyntarová (07.02.2024)

Seminars

 1.    Polymerase chain reaction (PCR) and its modifications (3hrs) – theory of PCR, both classical and advanced modifications

2.    Sequencing (3hrs) - theory of sequencing reactions, Sanger sequencing, NGS and their use in the microbiological diagnosis and research

3.    MALDI-TOF and its applications (3hrs) - principles and methodology of mass spectrometry

 

Practical classes

 1.    PCR - isolation of nucleic acids, setting up a PCR reaction, detection of products using gel electrophoresis (3-4hrs)

2.    MALDI-TOF - practically performing an identification of cultures (3hrs)

 

Lecturers:

• MUDr. Miroslav Fajfr, Ph.D.
• Mgr. Radka Bolehovská, Ph.D,
• Mgr. Rudolf Kukla, Ph.D.
• Mgr. Radek Sleha, Ph. D.

 

Literature

Last update: Eva Dyntarová (23.01.2024)

Recommended literature:

1. Goering R, et al. Mims' Medical Microbiology and Immunology, 6th Ed., Elsevier, 2018.
2. Greenwood D. Medical Microbiology, 18^th Ed., Elsevier, 2012.