Microbiology II - EA0105084

• Title: Mikrobiologie II
• Guaranteed by: Ústav mikrobiologie (14-510)
• Faculty: Faculty of Medicine in Pilsen
• Actual: from 2021
• Semester: winter
• Points: 5
• E-Credits: 5
• Examination process: winter s.:
• Hours per week, examination: winter s.:3/2, C+Ex [HT]
• Capacity: unknown / unknown (unknown)
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: deregister from the exam date if a requisite was not fulfilled
• Guarantor: RNDr. Karel Fajfrlík, Ph.D.
• Co-requisite : EA0103012, EA0104011, EA0104510
• Is co-requisite for: EA0108024, EA0108120, EA0107015, EA0110020, EA0108023, EA0108030, EA0108031, EA0107041

Course completion requirements

Credit: attendance on practicals with active participation (two absences tolerated,  more absences accepted only if substantiated by

medical certificate), written and practical test

Exam: oral form

Last update: Martínek Karel, RNDr., Ph.D. (05.10.2020)

Syllabus

Winter semester 2020/2021

 Lectures:

• Introduction, history of clinical microbiology, evolution of microbes, bacterial genetics (viruses, bacteria, parasites, mechanisms of horizontal gene transfer - transformation, transduction, conjugation, specific genetic elements - insertion sequences, transposons, integrons, etc.) 
• Review of medically important parasites (general parasitology, live cycles, vectors, transmission patterns, diagnostics (Trypanosoma spp., Leishmania spp., Trichomonas vaginalis, Giardia intestinalis, Entamobea histolytica, Plasmodium spp., Toxoplasma gondii, Paragonimus westermani, Schistosoma spp., spp., Taenia spp., Echinococcus spp., Ascaris lumbricoides, Enterobius vermicularis, Sarcoptes scabei)
• Review of clinically important  G+ bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Streptococcus spp., Enterococcus spp., Staphylococcus spp., Bacillus spp., Corynebacterium spp., etc.)
• Review of clinically important  G- bacteria  (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Neisseria spp., Haemophilus spp., Enterobacteriaceae, Vibrio spp., Campylobacter spp., Helicobacter spp., Pseudomonas and other non-fermenting rods, Legionella spp.)
• Review of clinically important  bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Mycobacterium spp., Nocardia spp., Actinomyces spp., Chlamydia spp., Mycoplasma spp., Rickettsia spp., anaerobic bacteria - review of the most important species, e.g. Clostridium difficile, C. perfringens, C. tetani, C. botulinum, Peptostreptococcus spp., Fusobacterium spp.)
• Antibiotics (basic groups of antibiotics, mechanism of action, molecules; beta-lactams, glycopeptides, polymyxins, amynoglykosides, quinolones, sulfonamides, chloramphenicol, etc.)
• Mechanisms and epidemiology of antibiotic resistance (mechanisms of bacterial resistence, clonal spread of resistant bacteria, horizontal transfer of genes - mobilisation of genes, mobile genetic elements, integrons, co-resistnace, cross-resistance) 
• Hospital-acquired infections (definition, examples of the most important pathogens, resistence bacteria as a causative agents of hospital-acquired infections - MRSA, VRE, resistant Gram-negative rods - production of extended-spectrum beta-lactamases, carbapenemases)
• Review of viral diseases 1 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; papillomaviruses, polyomaviruses, adenovirus, herpesviruses, poxviruses, picornaviruses, coronaviruses,  paramyxoviruses, influenza virus, rhabdoviruses, filoviruses, reoviruses, togaviruses and flaviviruses, togaviruses, bunyaviridae) 
• Review of viral diseases  2 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; retroviruses - HIV, hepatitis viruses)
• Indication, limitations, and interpretation of microbiological diagnostics methods

 

Practical courses:

5.10 - 9.10                Preanalytical Phase, Normal Microflora

12.10. - 16.10.        Laboratory Diagnosis  of Mycotic Infections

19.10. - 23.10.        Laboratory Diagnosis  of Parasitic Infections

26.10. – 30.10.       Laboratory Diagnosis of  Viral Infections

(28.10. public holiday)

2.11. - 6.11.            Laboratory Diagnosis of  Skin, Joints and  Bones Infections, Anaerobic Bacteria 

9.11. - 13.11.          Laboratory Diagnosis of Urinary Tract Infections, Laboratory Diagnosis of  Sexually Transmitted Diseases

16.11 - 20.11.         Test, Laboratory Diagnosis of Gastrointestinal Tract Infections

(17.11. public holiday)

23.11. - 27.11.        Laboratory Diagnosis of Respiratory Tract Infections

30.11. - 4.12.          Sepses and Bloodstream Infections

7.12. - 11.12.          Laboratory Diagnosis of CNS infections, Nosocomial Infections

14.12. - 18.12.        Antimicrobial Terapy - Case Reports

4.1. - 8.1.                Test, Credits

 

 

 

 

Last update: Martínek Karel, RNDr., Ph.D. (16.10.2020)

Course completion requirements

Credit: attendance on practicals with active participation (two absences tolerated,  more absences accepted only if substantiated by

medical certificate), written and practical test

Exam: oral form

Last update: Martínek Karel, RNDr., Ph.D. (05.10.2020)