OS - Tissue engineering and nanobiotechnology in regenerative medicine - DVA1096

• Title: OS - Tissue engineering and nanobiotechnology in regenerative medicine
• Guaranteed by: Department of Biophysics (13-714)
• Faculty: Second Faculty of Medicine
• Actual: from 2023
• Semester: winter
• Points: 3
• E-Credits: 3
• Examination process: winter s.:
• Hours per week, examination: winter s.:0/20, C [HS]
• Capacity: unknown / 18 (18)
• Min. number of students: 2
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: Mgr. Eva Filová, Ph.D.
• Teacher(s): prof. RNDr. Evžen Amler, CSc.; Mgr. Radek Divín; Mgr. Eva Filová, Ph.D.; Aleksei Pashchenko; Viktorie Ročková, M.Sc.

Course completion requirements

English

Credit - Students' attendance at 2 lectures and 2 practicals.

Last update: Kostohryzová Lenka (04.08.2023)

Czech

Credit: Attendance in lectures and practicals

Last update: Čechová Jana, Mgr. (13.09.2018)

Requirements to the exam

No exam

Last update: Heřman Petr, RNDr. (02.11.2017)

Syllabus

English

Introduction to tissue engineering

• Tissue structure – overview

• Selected techniques and methods applied in tissue and protein engineering – summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods)

• Regenerative medicine and tissue engineering

• Cells in tissue engineering – principle sources, cells isolated from tissues, embryonic and adult stem cells

Tissue engineering

• Cartilage tissue engineering

• Bone tissue engineering

• Skin covers

Nanofibers, their production and safety

• Nanofibers and production methods

• Safety of nanofibers and other nanosystems

• Medical textiles as scaffolds for cell seeding

• Reasons and methods of nanofiber functionalization

Bio-nanotechnology and systems of controlled drug release

• Application of bio-nanotechnology in tissue engineering and regenerative medicine

• Controlled drug delivery, liposomes, their preparation and application

• „Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules

Bio-nanotechnology for artificial tissue production

• Exogenous and endogenous cell sources

• Bio-nanotechnology and mobilization of endogenous cell sources

• Biomechanical properties on native and artificial tissues and their determination

• Quality testing of bio-implants (in vitro and in vivo tests)

Practicals– UCEEB CTU Martin Královič [e-mail]

Univerzitní centrum energeticky efektivních budov ČVUT v Praze

Laboratoř pokročilých biomateriálů

Třinecká 1024

273 43 Buštěhrad

A. Nanofibers and controlled drug delivery (UCEEB CTU)

Task 1: Preparation of nanofibers by electrospinning and forcespinning methods

Task 2: Visualization of nanofiber's morphology (SEM analyses)

Task 3: Demonstration of 3D printing method

Task 4: Evaluation of physico-chemical properties of nanofibres (FTIR analysis)

B. In vitro characterization of cells Institute of Experimental Medicine of the ASCR (IEM ASCR)

[e-mail]

Institute of Experimental medicine of the ASCR

IBC building

Vídeňská 1083

142 20 Praha 4

Task 1: Cell passaging

Task 2: Cell staining, confocal microscopy – DiOC6 and propidium iodide staining

Task 3: Cells metabolic activity testing – MTS test

Last update: Čechová Jana, Mgr. (08.09.2019)

Czech

Introduction to tissue engineering
Tissue structure - overview

Selected techniques and methods applied in tissue and protein engineering - summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods)

Regenerative medicine and tissue engineering

Cells in tissue engineering - principle sources, cells isolated from tissues, embryonic and adult stem cells

Tissue engineering
Cartilage tissue engineering

Bone tissue engineering

Blood-vessel tissue engineering

Skin covers

Nanofibers, their production and safety
Nanofibers and production methods

Safety of nanofibers and other nanosystems

Medical textiles as scaffolds for cell seeding

Reasons and methods of nanofiber functionalization

Bio-nanotechnology and systems of controlled drug release

Application of bio-nanotechnology in tissue engineering and regenerative medicine

Controlled drug delivery, liposomes, their preparation and application

„Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules

Bio-nanotechnology for artificial tissue production
Exogenous and endogenous cell sources

Bio-nanotechnology and mobilization of endogenous cell sources

Biomechanical properties on native and artificial tissues and their determination

Quality testing of bio-implants (in vitro and in vivo tests)

Experimental part
A. Nanofibers and controlled drug delivery (UCEEB CTU)
Task 1: Preparation of nanofibers by electrospinning and forcespinning methods

Task 2: Visualization of nanofiber's morphology (SEM analysis)

Task 3: Evaluation of physico-chemical properties of nanofibers (FTIR)

Task 4: Demonstration of 3D printing method

B. In vitro characterization of cells (IEM ASCR)
Task 1: Cell passaging

Task 2: Cell staining, confocal microscopy - DiOC6 and propidium iodide staining

Task 3: Cells metabolic activity testing - MTS test

Task 4: Visiting the GMP laboratory for human cell and tissue therapy at IBC ASCR

Last update: Čechová Jana, Mgr. (13.09.2018)