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Course, academic year 2023/2024
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OS - Tissue engineering and nanobiotechnology in regenerative medicine - DVA1096
Title: OS - Tissue engineering and nanobiotechnology in regenerative medicine
Guaranteed by: Department of Biophysics (13-714)
Faculty: Second Faculty of Medicine
Actual: from 2023
Semester: winter
Points: 3
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:0/20, C [HS]
Capacity: unknown / 18 (18)
Min. number of students: 2
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: English
Teaching methods: full-time
Teaching methods: full-time
Level:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Mgr. Eva Filová, Ph.D.
Course completion requirements -
Credit - Students' attendance at 2 lectures and 2 practicals.
Last update: Kostohryzová Lenka (04.08.2023)
Requirements to the exam

No exam

Last update: Heřman Petr, RNDr. (02.11.2017)
Syllabus -

Introduction to tissue engineering

• Tissue structure – overview

• Selected techniques and methods applied in tissue and protein engineering – summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods)

• Regenerative medicine and tissue engineering

• Cells in tissue engineering – principle sources, cells isolated from tissues, embryonic and adult stem cells

Tissue engineering

• Cartilage tissue engineering

• Bone tissue engineering

• Skin covers

Nanofibers, their production and safety

• Nanofibers and production methods

• Safety of nanofibers and other nanosystems

• Medical textiles as scaffolds for cell seeding

• Reasons and methods of nanofiber functionalization

Bio-nanotechnology and systems of controlled drug release

• Application of bio-nanotechnology in tissue engineering and regenerative medicine

• Controlled drug delivery, liposomes, their preparation and application

• „Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules

Bio-nanotechnology for artificial tissue production

• Exogenous and endogenous cell sources

• Bio-nanotechnology and mobilization of endogenous cell sources

• Biomechanical properties on native and artificial tissues and their determination

• Quality testing of bio-implants (in vitro and in vivo tests)

Practicals– UCEEB CTU Martin Královič martin_kralovic@labdemo.cz

Univerzitní centrum energeticky efektivních budov ČVUT v Praze

Laboratoř pokročilých biomateriálů

Třinecká 1024

273 43 Buštěhrad

A. Nanofibers and controlled drug delivery (UCEEB CTU)

Task 1: Preparation of nanofibers by electrospinning and forcespinning methods

Task 2: Visualization of nanofiber's morphology (SEM analyses)

Task 3: Demonstration of 3D printing method

Task 4: Evaluation of physico-chemical properties of nanofibres (FTIR analysis)

B. In vitro characterization of cells Institute of Experimental Medicine of the ASCR (IEM ASCR)

eva.filova@iem.cas.cz

Institute of Experimental medicine of the ASCR

IBC building

Vídeňská 1083

142 20 Praha 4

Task 1: Cell passaging

Task 2: Cell staining, confocal microscopy – DiOC6 and propidium iodide staining

Task 3: Cells metabolic activity testing – MTS test

Last update: Čechová Jana, Mgr. (08.09.2019)
 
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