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Last update: doc. RNDr. Daniel Rösel, Ph.D. (01.08.2013)
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Last update: doc. RNDr. Daniel Rösel, Ph.D. (01.08.2013)
Lehninger. Principles of Biochemistry, 3rd edition (2000), ISBN 1-57259-153-6, Nelson, D.L., Cox, M.M. Worth Publishers, New York Voet, D. and Voet, J.: Biochemistry. 3rd ed. (2004), ISBN 0-471-19350-x, John Wiley & Sons, Inc. |
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Last update: doc. RNDr. Daniel Rösel, Ph.D. (01.08.2013)
Conditions for obtaining credits: 1. Attendance on the introductory lecture and successful completion of the entrance exam. 2. Active participation in experimental work. 3. Written report that meets the requirements (before the beginning of summer semester). |
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Last update: doc. RNDr. Daniel Rösel, Ph.D. (24.02.2021)
Examples of experiments: 1) Determination of glycogen concentration in rat liver after starvation: the experimental aim is to isolate and determine glycogen content in rat liver. The glycogen content in the livers of two individuals, normally fed rats and short-term fasted rats, will be compared. Glycogen will be separated from mono- and oligosaccharides and hydrolyzed in acidic conditions. The resulting glucose will be determined spectrophotometrically using an enzymatic test. 2) Isolation of recombinant protein, SDS-PAGE electrophoresis: the experimental aim is to isolate GST-fusion protein (14-3-3 or beta-galactosidase) from a bacterial culture of Escherichia coli. Recombinant GST-fusion protein will be isolated from bacterial culture of E. coli by affinity chromatography. The quality of the individual purification steps will be verified by SDS-PAGE electrophoresis. 3) Phospholipids in biological membranes: the experimental aim is to compare the quantitatively different content of phospholipids in the membranes of erythrocytes of ruminant and non-ruminant animals. Phospholipids will be divided into basic groups by thin layer chromatography and the relative proportion of selected phospholipid species will be determined quantitatively by densitometric image analysis. |
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Last update: doc. RNDr. Daniel Rösel, Ph.D. (01.08.2013)
Zápis na úvodní přednášku a zápis do turnusů probíhá elektronicky na serveru MOODLE (http://dl2.cuni.cz/). Kurz samotný najdete v nabídce: Přírodovědecká fakulta, Sekce biologie, Katedra buněčné biologie, PBB. |