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Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)
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Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)
Relevant literature: M.P. Deutscher: Guide to Protein Purification. Academic Press 1990.
Detailed and up-to-date protocols will available for every projet during the course. |
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Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)
Active participation on the course, mastering of the methods, calculation of kinetic and molecular parameters of the studied proteins, 100% attendance. |
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Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)
1. Cell homogenization methods 2. Methods of cell fractionation, isolation of organelles 3. Principles of protein separation techniques 4. Enzyme activity determinations, kinetic measurements 5. Protein purification |