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Last update: RNDr. Ingrid Poláková, Ph.D. (22.08.2023)
interpretation of results. The methods will include extraction of nucleic acids from different type of clinical material. The students will analyze the quality and quantity of nucleic acids. They will also detect the selected genes by the polymerase chain reaction (PCR) and/or real time PCR. The specificity of amplicons will be confirmed by sequencing. For the detection of viral specific antibodies Enzyme-Linked Immunosorbent Assay (ELISA) and/or indirect immunofluorescence method will be used. The course will be held in BIOCEV, Vestec from Oct 23 to 27 |
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Last update: RNDr. Jana Šmahelová (06.09.2022)
See MB140P75 |
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Last update: RNDr. Ingrid Poláková, Ph.D. (22.08.2023)
The course will be held in BIOCEV, Vestec from Oct 23 to 27 In order to obtain credits the students have to provide the protocols per each group (2 students per group). The protocols have to include interpretation of the results. |
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Last update: RNDr. Ingrid Poláková, Ph.D. (29.08.2022)
Direct methods - PCR, qRT PCR, sequencing Indirect methods - imunofluorescence, ELISA
Emphasis will be on good clinical practice. The course will mimic the situation in the clinical laboratory. |