Stem Cells and Regenerative Medicine - FG50019

• Title: Stem Cells and Regenerative Medicine
• Guaranteed by: Department of Histology and Embryology (15-140)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2022
• Semester: summer
• Points: 0
• E-Credits: 2
• Examination process: summer s.:
• Hours per week, examination: summer s.:25/7, C [HT]
• Capacity: 35
• Min. number of students: 5
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: prof. MUDr. Jaroslav Mokrý, Ph.D.; MUDr. Tomáš Soukup, Ph.D.
• Incompatibility : FV045
• Pre-requisite : FG10004
• Interchangeability : FV045
• Is incompatible with: FV045
• Is interchangeable with: FV045

Annotation

English

Last update: Markéta Ulrichová (04.01.2022)

Students who have passed histology, embryology and biology are eligible to register. In lectures, students gain knowledge in a progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.

Czech

Last update: Markéta Ulrichová (04.01.2022)

Předmět navazuje na znalosti cytologie. Studenty seznamuje s diskutovanými tématy současnosti, jakými bezpochyby jsou: kmenové buňky, buněčné kultury v biomedicíně, buněčná terapie, regenerativní medicína, tkáňové inženýrství apod. V praktických cvičeních jsou studenti obeznámeni se základy práce v laboratoři tkáňových kultur a s detekčními metodami.

Requirements to the exam

Last update: Markéta Ulrichová (04.01.2022)

Credit

Presence in lectures and laboratory classes. Absences must be substituted by a written essay on the missed topic. Elaboration of protocol for cell viability.

Syllabus

Last update: Markéta Ulrichová (10.01.2022)

Lectures

Lecturers
  Prof. Jaroslav Mokry, M.D., Ph.D.
  Prof. Stanislav Filip, M.D., Ph.D.
  Assoc. Prof. Pavel Zak, M.D., Ph.D.
  Tomas Soukup, M.D., Ph.D.

 

1    Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research. (Mokry, 3)
2    Pluripotent stem cells, ES cells, iPS cells. Epithelial stem cells. Tumour stem cells. Muscle-derived stem cells; muscle regeneration. (Mokry, 3)

3    Neural stem cells and their niche. Regeneration of the nervous tissue. Spinal cord injury. (Mokry, 3) 
4    Endothelial progenitor cells. Haematopoietic stem cells. Haematological applications, bone marrow transplantation. (Mokry, 1; Filip, 1; Žák, 1) 

5    Mesenchymal stem cells. Dental pulp stem cells. Separation techniques. Cellular interactions. Growth factors. (Soukup, 3) 
6    Scaffolds (2D, 3D) - natural, artificial. Encapsulation. Nanotechnology. Cellular migration. Cell differentiation. Cell transplantation. Tissue engineering of bones and cartilages Orthopaedic applications. (Soukup, 3) 

 
Practical courses and seminars

Lecturers
  Tomas Soukup, M.D., Ph.D.
  Assoc. Prof. Jakub Suchanek, M.D., Ph.D.

 
Practical courses
1 Tissue engineering. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy.
2 Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics.
3 Cell isolation. Primary cultures. Subculturing. Cultivation surfaces. Basic biological characteristics. Cell separation techniques. Gradient centrifugation (Ficoll, Percoll). Immunomagnetic separation.
4 Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis.
5 Visit of tissue culture laboratory. Cell viability. Preparation of cells for flow cytometric analysis.

 

Literature

Last update: Markéta Ulrichová (04.01.2022)

Recommended  literature

1. Alberts et al Molecular Biology of the Cell, Garland, ISBN 0815340729
 
2. Sell, S. Stem Cell Handbook, Humana Press, 2004,4th Editoion,2002, E-ISBN 1-59259-411-5
 
3. Turksen, K. Adult Stem Cells, Humana Press, 2004, E-ISBN 1-59259-732-7
 
4. Clark et al. The Molecular and Cellular Biology of Wound Repair, Plenum Press, 1996, ISBN 030645159X
 
5. Doyle et al. Mammalian Cell Culture (Essential Techniques), John Wiley and Sons, 1997, ISBN 0-471-97057-3
 
6. Bronzino et al. The Biomedical Engineering Handbook, CRC Press, 1999
 
7. Ratner et al. Biomaterial Science, Academic Press, 1997, ISBN 012824610
 
8. Patrick et al. Frontiers in Tissue Engineering, Pergamon, 1998, ISBN 0080426891
 
9. Lanza et al. Principles of Tissue Engineering, Academic Press, 2000, ISBN 0124366309

10. Frank H. Stephenson Calculations for Molecular Biology and Biotechnology, Academic Press, Elsevier Science 2003, ISBN 0-12-665751-3