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Role jaderných laminů a Nup358 v infekci polyomavirem BK
| Thesis title in Czech: | Role jaderných laminů a Nup358 v infekci polyomavirem BK |
|---|---|
| Thesis title in English: | Role of nuclear lamins and Nup358 in BK polyomavirus infection |
| Key words: | BKPyV, jaderná lamina, Nup358, VP1, Rab7, LC3B, autofagozom |
| English key words: | BKPyV, nuclear lamina, Nup358, VP1, Rab7, LC3B, autophagosome |
| Academic year of topic announcement: | 2020/2021 |
| Thesis type: | diploma thesis |
| Thesis language: | čeština |
| Department: | Department of Genetics and Microbiology (31-140) |
| Supervisor: | Mgr. Kateřina Bruštíková, Ph.D. |
| Author: | hidden - assigned and confirmed by the Study Dept. |
| Date of registration: | 20.10.2020 |
| Date of assignment: | 20.10.2020 |
| Confirmed by Study dept. on: | 20.10.2020 |
| Date of electronic submission: | 26.04.2023 |
| Date of proceeded defence: | 30.05.2023 |
| Opponents: | RNDr. Šárka Němečková, DrSc. |
| Preliminary scope of work |
| Cílem práce je zjistit, zda v průběhu infekce hostitelských buněk lidským polyomavirem BK či myším polyomavirem dochází k změnám ve struktuře a modifikaci proteinů jaderného obalu. Důraz bude kladen zejména na zjištění případné interakce časných a pozdních proteinů polyomavirů s proteiny komplexu jaderného póru a s proteiny jaderné laminy a s nimi asociovanými proteiny. V průběhu práce budou použity jak biochemické metody, tak pokorčile mikroskopické techniky. |
| Preliminary scope of work in English |
| The aim of this work is to determine whether during the infection of host cells with human polyomavirus BK or mouse polyomavirus, there are changes in the structure and modification of nuclear envelope proteins. Emphasis will be placed on identifying the possible interaction of early and late polyomavirus proteins with nuclear pore complex proteins and with nuclear lamina proteins and associated proteins. During the work, both biochemical methods and advanced microscopic techniques will be used. |