Syntéza FeS center u amitochondriálního eukaryota
| Thesis title in Czech: | Syntéza FeS center u amitochondriálního eukaryota |
|---|---|
| Thesis title in English: | FeS cluster assembly in an amitochondriate eukaryote |
| Key words: | Amitochondriální eukaryota, syntéza FeS center, Suf system, oxymonády, mitochondrie, struktura proteinů |
| English key words: | Amitochondriate eukaryote, FeS cluster assembly, Suf system, oxymonads, mitochondrion, protein structure |
| Academic year of topic announcement: | 2019/2020 |
| Thesis type: | dissertation |
| Thesis language: | čeština |
| Department: | Department of Parasitology (31-161) |
| Supervisor: | doc. Mgr. Vladimír Hampl, Ph.D. |
| Author: |
| References |
| 1: Hampl V, Čepička I, Eliáš M. Was the Mitochondrion Necessary to Start Eukaryogenesis? Trends Microbiol. 2019 Feb;27(2):96-104. doi: 10.1016/j.tim.2018.10.005. Epub 2018 Nov 19. PMID: 30466901. 2: Vacek V, Novák LVF, Treitli SC, Táborský P, Cepicka I, Kolísko M, Keeling PJ, Hampl V. Fe-S Cluster Assembly in Oxymonads and Related Protists. Mol Biol Evol. 2018 Nov 1;35(11):2712-2718. doi: 10.1093/molbev/msy168. PMID: 30184127; PMCID: PMC6231488. 3: Karnkowska A, Hampl V. The curious case of vanishing mitochondria. Microb Cell. 2016 Sep 30;3(10):491-494. doi: 10.15698/mic2016.10.531. PMID: 28357316; PMCID: PMC5348982. 4: Karnkowska A, Vacek V, Zubáčová Z, Treitli SC, Petrželková R, Eme L, Novák L, Žárský V, Barlow LD, Herman EK, Soukal P, Hroudová M, Doležal P, Stairs CW, Roger AJ, Eliáš M, Dacks JB, Vlček Č, Hampl V. A Eukaryote without a Mitochondrial Organelle. Curr Biol. 2016 May 23;26(10):1274-84. doi: 10.1016/j.cub.2016.03.053. Epub 2016 May 12. PMID: 27185558. |
| Preliminary scope of work |
| Jak funguje eukaryot bez mitochondrií? Jak jsou syntetizovány základní kofaktory proteinů, Fe-S centra? K vyřešení hádanky, jak evoluce eliminovala mitochondrie z prvoka Monocercomonoides exilis, používáme molekulární a biochemické techniky. Při práci na projektu se naučíte molekulární klonování a expresi proteinů v prokaryotech a eukaryotech, kultivace, imunofluorescenční mikroskopii, imunoprecipitaci a další techniky. Neváhejte nás kontaktovat! |
| Preliminary scope of work in English |
| How does a eukaryote function without mitochondria? How are the essential cofactors of proteins, Fe-S clusters, synthesised? We use molecular and biochemical techniques to solve the riddle of how evolution eliminated mitochondria from Monocercomonoides exilis. While working on the project, you would learn molecular cloning and protein expression in prokaryotes and eukaryotes, cell culture, immunofluorescence microscopy, immunoprecipitation amongst others. Do not hesitate to contact us for a chat! |