Bobek, J., Filipova, E., Bergman, N., Cihak, M., Petricek, M., Lara, A.C., Kristufek, V., Megyes, M., Wurzer, T., Chronakova, A., and Petrickova, K. (2022). Polyenic Antibiotics and Other Antifungal Compounds Produced by Hemolytic Streptomyces Species. Int J Mol Sci 23. Herbrik, A., Corretto, E., Chronakova, A., Langhansova, H., Petraskova, P., Hrdy, J., Cihak, M., Kristufek, V., Bobek, J., Petricek, M., and Petrickova, K. (2019). A Human Lung-Associated Streptomyces sp. TR1341 Produces Various Secondary Metabolites Responsible for Virulence, Cytotoxicity and Modulation of Immune Response. Front Microbiol 10, 3028. Kotrbová, L., Lara, A.C., Corretto, E., Scharfen, J., Ulmann, V., Petříčková, K., and Chroňáková, A. (2022). Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns. Sci Rep 12, 9353.
Preliminary scope of work
Projekt se zaměří na výzkum streptomycet jako zdroje antimykotických látek s cílem eliminace multirezistentních mikromycet. Bude provedena analýza komenzálních streptomycet, identifikace aktivních látek (ve spolupráci s Laboratoří antibiotické rezistence a mikrobiální metabolomiky MBÚ AV ČR), jejich strukturní charakterizace a hodnocení cytotoxicity (ve spolupráci s Farmakologickým ústavem 1. LF UK). Projekt zahrnuje kultivace streptomycet a jejich ko-kultivace s mikromycetami, fenotypovou analýzu, izolaci a charakterizaci produkovaných látek a přípravu vědeckých publikací.
Preliminary scope of work in English
The project will focus on the research of streptomycetes as a source of antifungal compounds aimed at eliminating multidrug-resistant micromycetes. The study will include an analysis of commensal streptomycetes, identification of active compounds (in collaboration with the Laboratory of Antibiotic Resistance and Microbial Metabolomics of the Czech Academy of Sciences), structural characterization of these compounds, and assessment of their cytotoxicity (in collaboration with the Institute of Pharmacology at the First Faculty of Medicine, Charles University). The project encompasses cultivation of streptomycetes and their co-cultivation with micromycetes, phenotypic analysis, isolation and characterization of produced compounds, and preparation of scientific publications.
